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首页> 外文期刊>Biochemical Systematics and Ecology >A genetic diversity study of silkworm using cleaved amplified polymorphic sequence (CAPS) markers.
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A genetic diversity study of silkworm using cleaved amplified polymorphic sequence (CAPS) markers.

机译:利用裂解的扩增多态性序列(CAPS)标记对家蚕进行遗传多样性研究。

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摘要

The silkworm, Bombyx mori is a beneficial insect of great economic importance in China for its silk production. In this study, we obtained 11 cleaved amplified polymorphic sequence (CAPS) markers and one PCR polymorphism marker from the genes of the silkworm, B. mori. A backcross progeny analysis showed that all these molecular markers were segregated in a Mendelian fashion and that polymorphisms were co-dominant. These markers were used to investigate the genetic diversity among 29 strains of B. mori from China, Japan and Europe. Cluster analysis, based on the genetic similarities calculated from CAPS data, grouped these strains roughly according to their geographical origin. One group contained silkworm strains from Europe and some of the Japanese strains were interspersed into the Chinese groups, whereas other Japanese strains clustered together..
机译:家蚕是一种有益的昆虫,对中国的蚕丝生产具有重要的经济意义。在这项研究中,我们从家蚕的基因中获得了11个裂解的扩增多态性序列(CAPS)标记和一个PCR多态性标记。回交的后代分析表明,所有这些分子标记均以孟德尔方式分离,并且多态性是共同优势。这些标记用于研究29种来自中国,日本和欧洲的桑蚕的遗传多样性。基于从CAPS数据计算出的遗传相似性进行的聚类分析,将这些菌株大致按其地理起源进行了分组。一组包含来自欧洲的蚕品系,一些日本品系散布在中国组中,而其他日本品系聚集在一起。

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