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首页> 外文期刊>Evidence-based complementary and alternative medicine: eCAM >The Antibacterial Effect of Ethanol Extract of Polish Propolis on Mutans Streptococci and Lactobacilli Isolated from Saliva
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The Antibacterial Effect of Ethanol Extract of Polish Propolis on Mutans Streptococci and Lactobacilli Isolated from Saliva

机译:波兰蜂胶乙醇提取物对唾液中变形链球菌和乳酸菌的抗菌作用

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Dental caries occurrence is caused by the colonization of oral microorganisms and accumulation of extracellular polysaccharides synthesized by Streptococcus mutans with the synergistic influence of Lactobacillus spp. bacteria. The aim of this study was to determine ex vivo the antibacterial properties of ethanol extract of propolis (EEP), collected in Poland, against the main cariogenic bacteria: salivary mutans streptococci and lactobacilli. The isolation of mutans streptococci group bacteria (MS) and Lactobacillus spp. (LB) from stimulated saliva was performed by in-office CRT bacteria dip slide test. The broth diffusion method and AlamarBlue assay were used to evaluate the antimicrobial activity of EEP, with the estimation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The biochemical composition of propolis components was assessed. The mean MIC and MBC values of EEP, in concentrations ranging from 25 mg/mL to 0.025 mg/mL, for the MS and LB were found to be 1.10 mg/mL versus 0.7 mg/mL and 9.01 mg/mL versus 5.91 mg/mL, respectively. The exposure to an extract of Polish propolis affected mutans streptococci and Lactobacillus spp. viability, exhibiting an antibacterial efficacy on mutans streptococci group bacteria and lactobacilli saliva residents, while lactobacilli were more susceptible to EEP. Antibacterial measures containing propolis could be the local agents acting against cariogenic bacteria.
机译:龋齿的发生是由于口腔微生物的定殖和变形链球菌合成的细胞外多糖的积累,并具有乳酸杆菌属的协同作用。菌。这项研究的目的是确定从波兰收集的蜂胶乙醇提取物(EEP)对主要的致癌细菌:唾液变形链球菌和乳杆菌的抗菌特性。变形链球菌群细菌(MS)和乳酸杆菌属的分离。通过办公室内CRT细菌浸入玻片试验从受刺激的唾液中提取(LB)。肉汤扩散法和AlamarBlue分析法用于评估EEP的抗菌活性,并估计其最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。评估蜂胶成分的生化组成。对于MS和LB,EEP的平均MIC和MBC值范围为25 mg / mL至0.025 mg / mL,发现分别为1.10 mg / mL,0.7 mg / mL和9.01 mg / mL与5.91 mg / mL。 mL,分别。暴露于波兰蜂胶提取物会影响变形链球菌和乳酸杆菌属。活力,对变形链球菌群细菌和唾液乳杆菌居民表现出抗菌作用,而乳杆菌对EEP更敏感。含有蜂胶的抗菌措施可能是对抗龋齿细菌的局部药物。

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