• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Expert opinion on biological therapy >The use of fibrin based matrices and fibrin microbeads (FMB) for cell based tissue regeneration.
【24h】

The use of fibrin based matrices and fibrin microbeads (FMB) for cell based tissue regeneration.

机译：使用基于纤维蛋白的基质和基于纤维蛋白的微珠（FMB）进行基于细胞的组织再生。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

BACKGROUND: Due to its good cell attachment capabilities and promotion of cell migration, fibrin serves as an interim cell-binding matrix in wounded tissues. Due to their fast degradation, unprocessed fibrin matrices have limited use in tissue engineering. OBJECTIVE: To describe stable fibrin-based matrices for isolation, growth and delivery of stem cells for implantation to enhance tissue regeneration. METHODS: Fibrin microbeads (FMB) were produced by moderate-heat condensation of fibrin particles in oil without compromising the cell binding capability of the fibrin. RESULTS: Mesenchymal stem cells (MSC) were separated from different sources at much higher yields with FMB. They were further expanded on them in suspension without trypsinization and passages. Cells on FMB could be induced to differentiate into different phenotypes, such as bone and cartilage. This enabled implantation of the cells on FMB for cell-based tissue regeneration. CONCLUSIONS: FMB technology provides a simple and effective method for cell separation, expansion in suspension and delivery for tissue regeneration.
机译：背景：由于其良好的细胞附着能力和促进细胞迁移，血纤维蛋白在受伤的组织中充当临时的细胞结合基质。由于它们的快速降解，未加工的纤维蛋白基质在组织工程中的使用受到限制。目的：描述稳定的基于纤维蛋白的基质，用于干细胞的分离，生长和输送，以促进组织再生。方法：在不损害血纤蛋白的细胞结合能力的情况下，将血纤蛋白颗粒在油中适度热缩合可产生血纤蛋白微珠（FMB）。结果：使用FMB从不同来源分离间充质干细胞（MSC），产率更高。它们在没有胰蛋白酶消化和传代的情况下在悬浮液上进一步扩增。可以诱导FMB上的细胞分化为不同的表型，例如骨骼和软骨。这样就可以将细胞植入FMB，以进行基于细胞的组织再生。结论：FMB技术提供了一种简单有效的方法来进行细胞分离，悬浮扩增和组织再生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号