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PCR based molecular characterization of Nepenthes khasiana Hook. f.pitcher plant

机译：基于PCR的哈萨那猪笼草的分子表征。皮彻植物

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摘要

Nepenthes khasiana Hook. f. belonging to monotypic family Nepenthaceae is a rare, endangered, dioecious member of the carnivorous plant found in North-East India. The plant is endemic to the Indian state of Meghalaya and is distributed throughout the state from West Khasi hills to East Khasi hills, Jaintia hills and East, West to South Garo hills from 1,000 to ca. 1,500 m altitude. Multi-locus analysis using PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers were used for the first time to assess the genetic diversities of N. khasiana Hook. f. collected from different parts of Meghalaya. It was observed that RAPD analysis showed more polymorphism than ISSR fingerprinting in revealing genetic polymorphism in N. khasiana Hook. f. The result of cluster analysis by using UPGMA method showed that the groups based on pooled RAPD-ISSR genetic similarity were more similar than the groups based on RAPD. Furthermore, genetic similarity reveals variability within the population at Jarain of Jaintia hills, while between populations the Baghmara region differs from the others with at least 40% dissimilarity. The results show a broad range of genetic diversity within the populations of N. khasiana Hook. f.
机译：猪笼草khasiana钩。 F。属于Nepenthaceae的单型科，是印度东北部发现的一种食肉植物的稀有，濒危，雌雄异体的成员。该植物是印度梅加拉亚邦特有的植物，分布在整个州，从西卡西丘陵到东卡西丘陵，Jaintia丘陵，从东，西到南加洛丘陵从1000到ca。海拔1500 m。首次使用基于PCR的随机扩增多态性DNA（RAPD）和内部简单序列重复（ISSR）标记进行多基因座分析，以评估哈萨克猪笼草的遗传多样性。 F。从梅加拉亚邦的不同地方收集。观察到，RAPD分析显示出比哈苏猪笼草遗传多态性更多的多态性，而不是ISSR指纹图谱。 F。使用UPGMA方法进行聚类分析的结果表明，基于汇集的RAPD-ISSR遗传相似性的组比基于RAPD的组更相似。此外，遗传相似性揭示了Jaintia丘陵Jarain地区种群内部的变异性，而Baghmara地区之间的种群之间至少有40％的差异。结果表明，在哈萨克斯坦胡克猪笼草种群中存在广泛的遗传多样性。 F。

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