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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic structure of highland papayas (Vasconcellea pubescens (Lenn, et C. Koch) Badillo) cultivated along a geographic gradient in Chile as revealed by Inter Simple Sequence Repeats (ISSR)
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Genetic structure of highland papayas (Vasconcellea pubescens (Lenn, et C. Koch) Badillo) cultivated along a geographic gradient in Chile as revealed by Inter Simple Sequence Repeats (ISSR)

机译：如国际简单重复序列（ISSR）所示，智利沿地理梯度种植的高地木瓜（Vasconcellea pubescens（Lenn，C. Koch）Badillo）的遗传结构

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摘要

In Chile Vasconcellea pubescens is cropped to produce canned fruit, juice, jam and processed sweets. Additionally this species produces latex with a high level of papain, an important and valuable proteolytic enzyme with industrial applications. In this investigation seven ISSR primers were used to study the level and organization of genetic diversity in 333 samples of V. pubescens. Out of the 114 bands recorded, 63 proved to be polymorphic (P = 55.3%). At the species level, the genetic diversity was rather low (h = 0.01 +/- A 6,80188E-05, Shannon's Index I = 0.16 +/- A 0,000148). The major portion of the genetic diversity was found within groups (65%). The genetic differentiation between the different groups was significant, as the AMOVA analysis suggested (I broken vertical bar(pt) = 0.35). When analysing the Northern area alone, the differentiation increased to I broken vertical bar(pt) = 0.40. When only the Southern area was analysed, I broken vertical bar(pt) decreased to 0.18, indicating greater genetic similarity among the samples. The results generated from Structure and Bayesian Analysis of Population Structure distinguished 8 genetically different groups, five of them located in the north and three in the south. The results are discussed in the light of the growers' practices.
机译：在智利，Vasconcellea pubescens被种植来生产水果罐头，果汁，果酱和加工的糖果。此外，该物种生产的木瓜蛋白酶含量高，是工业应用中一种重要且有价值的蛋白水解酶。在这项调查中，使用了7种ISSR引物来研究333个耻骨葡萄样品的遗传多样性水平和组织。在记录的114个频段中，有63个被证明是多态的（P = 55.3％）。在物种水平上，遗传多样性非常低（h = 0.01 +/- A 6,80188E-05，香农指数I = 0.16 +/- A 0,000148）。遗传多样性的主要部分是在群体中发现的（65％）。正如AMOVA分析所表明的那样，不同组之间的遗传分化是显着的（我打破了竖线（pt）= 0.35）。仅分析北部地区时，差异增加到垂直折线（pt）= 0.40。当仅分析南部地区时，我打破的竖线（pt）降低至0.18，表明样品之间的遗传相似性更高。结构和贝叶斯人口结构分析得出的结果区分了8个遗传不同的群体，其中五个位于北部，三个位于南部。将根据种植者的做法对结果进行讨论。

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