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首页> 外文期刊>Genetic Resources and Crop Evolution >Viability of Oryza sativa L. seeds stored under genebank conditions for up to 30 years
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Viability of Oryza sativa L. seeds stored under genebank conditions for up to 30 years

机译：在基因库条件下保存长达30年的水稻种子的活力

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Germination ability, equilibrium relative humidity (eRH), and moisture content of 'control' seed samples representing 183 rice accessions stored in the active (2-4 A degrees C) and base (-10 A degrees C until 1993, then -20 A degrees C) collections of the T. T. Chang Genetic Resources Center were determined after storage for 20.5-30.5 years. Germination of seeds that had been stored in the base collection was generally high (> 70 %), whereas germination was more variable for seeds stored in the active collection. Samples with lower viability after storage in the active collection were likely to have lower viability after storage in the base collection. There were significant differences in the moisture content-eRH relationship of the seeds depending on whether the seeds had been stored in the active or base collection. Based on re-test data for regular seed samples regenerated in 1979-1980 and stored in the active collection for up to 31 years, estimates of the time for ability to germinate to fall to 50 % (p (50)) ranged from 54 to 997 years. For the same seed samples stored in the base collection for approximately 31 years, ability to germinate has been maintained and germination increased due to improved procedures. The ability to germinate of base collection samples was also generally higher than that of 'safety duplicate' samples of the same seed lots that had been sent to the National Center for Genetic Resources Preservation, USA in 1981 and stored at -18 A degrees C. This may have been due to uptake of moisture either during processing for dispatch or as a consequence of poor packaging material. The results are discussed in relation to long-term seed storage and genebank management.
机译：发芽能力，平衡相对湿度（eRH）和“对照”种子样品的水分含量，代表183种水稻的活性成分（2-4 A摄氏度）和基础（-10 A摄氏度，直到1993年，然后-20 A储藏期20.5-30.5年后确定TT Chang遗传资源中心的C）收集品。基本贮藏中种子的发芽率通常较高（> 70％），而有效贮藏中种子的发芽率变化更大。在活性收集物中储存后活力较低的样品在基础收集物中储存后活力较低。种子的水分含量与eRH的关系存在显着差异，具体取决于种子是存储在活性物质库中还是贮藏在碱库中。根据1979年至1980年间再生的常规种子样品的再测试数据，并在活跃的样本库中存储长达31年，发芽能力下降到50％的时间估计值（p（50））为54至997年。对于存储在基本集合中约31年的相同种子样品，由于改进了程序，发芽能力得以维持，发芽量增加。基本收集样品的发芽能力通常也比1981年发送到美国国家遗传资源保护中心并储存在-18 A摄氏度的相同种子批次的“安全重复”样品高。这可能是由于在发送处理过程中吸收了水分，或者是由于包装材料不良所致。讨论了有关种子长期保存和种质库管理的结果。

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