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首页> 外文期刊>Genetica >Identification and distribution of Puroindoline b-2 variant gene homologs in Hordeum
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Identification and distribution of Puroindoline b-2 variant gene homologs in Hordeum

机译：大麦中Puroindoline b-2变异基因同源物的鉴定和分布

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The barley hordoindoline genes (Hina and Hinb) are homologous to the wheat puroindoline genes (Pina and Pinb). These genes are involved in grain hardness, which is an important quality for barley processing. We identified novel variants of Hina and Hinb in 10 wild Hordeum species (H. bogdanii, H. brachyantherum, H. bulbosum, H. chilense, H. comosum, H. marinum, H. murinum, H. patagonicum, H. pusillum, and H. roshevitzii) covering all Hordeum genomes and preliminarily named them Hinc. These nucleotide sequences were highly similar to those of Puroindoline b-2 variant genes (Pinb-2v) and were located on chromosome 7I in H. chilense. The Hinc genes in H. bogdanii, H. bulbosum, H. patagonicum, and H. roshevitzii were pseudogenes possessing in-frame stop codons. We also found a partial Hinc sequence in H. murinum. This gene was not found in cultivated barley and H. vulgare subsp. spontaneum. The phylogenetic tree of Gsp-1, Hin, and Pin genes demonstrates that Hinc and Pinb-2v genes formed one cluster. Therefore, we considered that Hinc and Pinb-2v genes shared a common ancestral gene and were homologous to each other. We also studied the evolutional process of Gsp-1, Hin, and Pin genes. Our results suggested that Gsp-1 might be the most closely related to a putative ancestral gene on Ha locus.
机译：大麦Hordoindoline基因（Hina和Hinb）与小麦puroindoline基因（Pina和Pinb）同源。这些基因与谷物硬度有关，谷物硬度是大麦加工的重要品质。我们在10个野生大麦树种（H. bogdanii，H。brachyantherum，H。bulbosum，H。chilense，H。comosum，H。marinum，H。murinum，H。patagonicum，H。pusillum，和H. roshevitzii）涵盖了所有大麦的基因组，并初步命名为Hinc。这些核苷酸序列与Puroindoline b-2变异基因（Pinb-2v）高度相似，位于Chilense的7I染色体上。 H. bogdanii，H。bulbosum，H。patagonicum和H. roshevitzii中的Hinc基因是具有框内终止密码子的假基因。我们还发现了H. murinum中的部分Hinc序列。在栽培的大麦和普通小麦亚种中未发现该基因。自发性。 Gsp-1，Hin和Pin基因的系统发育树表明，Hinc和Pinb-2v基因形成一个簇。因此，我们认为Hinc和Pinb-2v基因共享一个共同的祖先基因，并且彼此同源。我们还研究了Gsp-1，Hin和Pin基因的进化过程。我们的研究结果表明，Gsp-1可能与Ha基因座上一个推测的祖先基因关系最密切。

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