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首页> 外文期刊>Genetic Resources and Crop Evolution >Spanish melons (Cucumis melo L.) of the Madrid provenance: a unique germplasm reservoir.
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Spanish melons (Cucumis melo L.) of the Madrid provenance: a unique germplasm reservoir.

机译:马德里起源的西班牙瓜( Cucumis melo L.):独特的种质库。

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摘要

Melon (Cucumis melo L.) landraces of the Madrid provenance, Spain, have received national distinction for their high fruit quality and sensorial attributes. More specifically, a unique array of Group Inodorus landraces have been continuously cultivated and conserved by farmers in the municipality of Villaconejos since the 19th century. Their genetic relationships to other Group Inodorus and Flexuous melon market classes is not known, and, thus, a study was designed to determine their genetic relationships using 52 simple sequence repeat (SSR) markers, and then make genetic comparisons between these accessions and a previously published "Standard Reference Germplasm Array" (RA) containing Group Inodorus (14 Spanish and one USA), Flexuosus (1 Spanish), and Cantalupensis (2 USA) melon accessions. This subset consisted of 15 Spanish Group Inodorus landraces that circumscribed the genetic variation of major Spanish melon market classes (Groups Inodorus and Flexuosus), and USA commercial varieties (Groups Cantalupensis and Inodorus). Based on genetic distances, Villaconejos (Madrid) genotypes differed substantially from RA subset accessions, thus defining their genetic uniqueness. Principal component analysis (PCA) partitioned the accessions examined into four distinct groups revealing that Villaconejos black epidermis melons (landraces 'Largo', 'Largo Negro Escrito' and 'Puchero') were distinctly different from all other accessions examined, as cluster analysis separated Rochet market type Villaconejos' accessions (landraces 'Mochuelo', 'Mochuelo Tradicional' and 'Melon de Villaconejos') from RA of the same market type. Genetic assessment of principal Spanish market classes revealed comparatively low intra-market heterogeneity in Piel de Sapo type accessions and high heterogeneity in Black and Yellow market type accessions. While a relatively high level of genetic introgression was detected between Yellow and Green market types, black epidermis market types were genetically unique. Given the uniqueness and high genetic diversity resident in Villaconejos landraces, this germplasm pool should be considered as a genetic source for broadening the comparatively narrow genetic base of Group Cantalupensis and Inodorus melon market types, especially standard commercial Spanish Group Inodorus market types (e.g., Piel de Sapo, Rochet, and Canari).Digital Object Identifier http://dx.doi.org/10.1007/s10722-011-9687-4
机译:西班牙马德里起源的甜瓜( L.)地方品种因其高品质的水果品质和感官特性而享誉全国。更具体地说,自19世纪以来,维拉康涅荷斯市的农民就在不断种植和养护独特的Inodorus集团地方品种。它们与其他Inodorus群体和柔韧性瓜市场类别的遗传关系尚不清楚,因此,一项研究旨在使用52个简单序列重复(SSR)标记物确定其遗传关系,然后对这些种质与以前的种质之间进行遗传比较。出版了“标准参考种质阵列”(RA),其中包含Inodorus组(14个西班牙文和一个美国),Flexuosus(1个西班牙文)和Cantalupensis(2个美国)甜瓜。该子集由15个西班牙Inodorus组地方品种组成,这些品种限制了西班牙主要瓜类市场类别(Inodorus和Flexuosus组)和美国商业品种(Cantalupensis和Inodorus组)的遗传变异。基于遗传距离,Villaconejos(Madrid)基因型与RA亚群登录号有显着差异,从而定义了它们的遗传唯一性。主成分分析(PCA)将检查的种质划分为四个不同的组,揭示了Villaconejos黑色表皮瓜(地方品种“ Largo”,“ Largo Negro Escrito”和“ Puchero”)与所有其他被种质明显不同,因为聚类分析将罗切特分开市场类型Villaconejos的登录名(本地品种“ Mochuelo”,“ Mochuelo Tradicional”和“ Melon de Villaconejos”)来自相同市场类型的RA。对西班牙主要市场类别的遗传评估显示,Piel de Sapo类型登录品的市场内异质性相对较低,而黑色和黄色市场类型登录品的异质性较高。虽然在黄色和绿色市场类型之间检测到较高水平的基因渗入,但黑表皮市场类型在基因上是独特的。考虑到Villaconejos地方品种的独特性和高遗传多样性,应考虑将该种质库作为拓宽Cantalupensis组和Inodorus瓜类市场类型,尤其是西班牙标准商业Inodorus组(例如Piel)市场类型的遗传来源的遗传资源。 de Sapo,Rochet和Canari)。数字对象标识符http://dx.doi.org/10.1007/s10722-011-9687-4

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