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Development, characterization, and variability analysis of microsatellites from a commercial cultivar of Musa acuminata

机译:商业化Musa acuminata栽培品种的微卫星的开发,表征和变异性分析

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摘要

We report the sequence and variability parameters of 23 microsatellite primers obtained from a commercial cultivar Gongjiao (Musa acuminata) using selectively amplified microsatellite (SAM) analysis. Polymorphisms were evaluated in a collection of 26 banana cultivars and 11 related species/subspecies. The mean number of alleles amplified per primer was 4.55 (range, 2-9), with a total of 100 alleles identified. The mean PIC value was 0.48 (range, 0.10-0.74). In addition, 22 markers also showed robust cross-species/genera amplification across 11 related species/subspecies, with the exception of Xiangtuijiao' (Ensete glaucum). Unweighted pair-grouping method with arithmetic averages (UPGMA) cluster analysis divided all the banana accessions into three main groups. The results demonstrate the usefulness of microsatellites for identification, similarity studies, and germplasm conservation in banana and related species.
机译:我们报告了23种微卫星引物的序列和变异性参数,这些引物是使用选择性扩增的微卫星(SAM)分析从商业栽培的龚角(Musa acuminata)获得的。在26个香蕉品种和11个相关物种/亚种的集合中评估了多态性。每个引物扩增的等位基因平均数为4.55(范围2-9),共鉴定出100个等位基因。 PIC平均值为0.48(范围为0.10-0.74)。此外,22个标记物在11个相关物种/亚种中也显示出强大的跨物种/属扩增,除了“相思鸟”(Ensete glaucum)。具有算术平均值的非加权对分组方法(UPGMA)聚类分析将所有香蕉种分为三个主要组。结果证明了微卫星对于香蕉和相关物种的鉴定,相似性研究和种质保存的有用性。

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