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首页> 外文期刊>Genes to cells : >Yeast Whi2 and Psr1-phosphatase form a complex and regulate STRE-mediated gene expression.
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Yeast Whi2 and Psr1-phosphatase form a complex and regulate STRE-mediated gene expression.

机译：酵母Whi2和Psr1-磷酸酶形成复合物并调节STRE介导的基因表达。

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BACKGROUND: In response to various stressful situations, including diauxic conditions, the Msn2 and Msn4 transcription factors induce STRE-mediated gene expression of many stress responsive genes in Saccharomyces cerevisiae. This is called the general stress response. The whi2 cells in the stationary phase are smaller than wild-type cells.RESULTS: Here we demonstrate that STRE-mediated gene expression in whi2 cells is reduced to half of that in the wild-type cells under various stress conditions. It is also delayed for several hours when the mutant cells enter the stationary phase. Using the two-hybrid system, we isolated a WHI2-interacting gene, PSR1, which is one of the redundant genes encoding plasma membrane phosphatases. whi2 and psr1 psr2 mutants had similar phenotypes, including reduced STRE-mediated gene expression, higher sensitivity to sodium ions and heat shock, and hyper-phosphorylation of Msn2. The phosphatase activity of Psr1 was necessary for the full activation of STRE-mediated gene expression. Furthermore, both Psr1 and Msn2 were co-immunoprecipitated with Whi2.CONCLUSIONS: Thus, Whi2 and its binding partner, Psr1-phosphatase, are required for a full activation of the general stress response, possibly through the dephosphorylation of Msn2. These results may explain why stationary phase whi2 cells are small.
机译：背景：针对各种压力情况，包括双生条件，Msn2和Msn4转录因子诱导酿酒酵母中许多应激反应基因的STRE介导的基因表达。这称为一般压力反应。结果：在这里，我们证明了在各种胁迫条件下，STRE介导的whi2细胞中的基因表达降低到野生型细胞中的一半。当突变细胞进入固定相时，它也延迟了几个小时。使用两个杂交系统，我们分离了一个WHI2相互作用基因PSR1，这是编码质膜磷酸酶的冗余基因之一。 whi2和psr1 psr2突变体具有相似的表型，包括减少的STRE介导的基因表达，对钠离子和热休克的更高敏感性以及Msn2的过度磷酸化。 Psr1的磷酸酶活性是完全激活STRE介导的基因表达所必需的。此外，Psr1和Msn2均与Whi2共免疫沉淀。结论：因此，Whi2及其结合伴侣Psr1-磷酸酶可能需要通过Msn2的去磷酸化才能完全激活一般应激反应。这些结果可以解释为什么固定相whi2细胞很小。

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