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首页> 外文期刊>Bulletin of the European Association of Fish Pathologists >Efficacy and immune response of intraperitoneal vaccination of rainbow trout (Oncorhynchus mykiss) with a Yersinia ruckeri bacterin formulated with Montanide (TM) ISA 763 AVG adjuvant
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Efficacy and immune response of intraperitoneal vaccination of rainbow trout (Oncorhynchus mykiss) with a Yersinia ruckeri bacterin formulated with Montanide (TM) ISA 763 AVG adjuvant

机译：用Montanide（TM）ISA 763 AVG佐剂配制的虹膜耶尔森菌腹膜内注射虹鳟鱼（Oncorhynchus mykiss）的功效和免疫反应

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摘要

Yersiniosis is an economically important bacterial disease affecting the aquaculture industry. Some immune response parameters and efficacy of intraperitoneal (IP) injection of Yersinia ruckeri bacterin (biotype I) formulated with Montanide ISA 763 AVG adjuvant were assessed in 100 g rainbow trout during 2-12 weeks post-vaccination. Fish were immunised by IP injection with the bacterin (ca 1 x 10(7) cfu/fish) of a virulent strain of Y. ruckeri with or without adjuvant and kept at 14 degrees C. Both cellular and humoral immune responses consisting of leukocyte count, lymphocyte population, lysozyme level, alternative hemolytic complement activity (ACH(50)) and antibody titer in fish immunised with the bacterin containing Montanide were higher than in fish immunised with the bacterin alone. However, these differences were not consistently significant between the two groups. The relative percent survival (RPS) up to 12 weeks post-immunisation reached 85-100% in fish immunised with the adjuvanted formulation while that of fish immunised with the bacterin alone was in the range of 75-90%. Our data showed that Montanide ISA 763 AVG used as an adjuvant in injectable Y. ruckeri vaccines can enhance both survival and immune response of rainbow trout.
机译：耶尔森氏病是影响水产养殖业的经济上重要的细菌疾病。在接种疫苗后的2至12周内，在100 g虹鳟鱼中评估了用Montanide ISA 763 AVG佐剂配制的腹膜内注射IP耶尔森氏小肠耶尔森氏菌（生物型I）的免疫应答参数和功效。通过IP注射鱼的致病性耶尔森氏菌强毒株（含或不含佐剂）的细菌素（ca 1 x 10（7）cfu /鱼），将其免疫，并保持在14摄氏度下。细胞和体液免疫应答均由白细胞计数组成，用蒙坦尼德菌苗免疫的鱼的淋巴细胞数量，溶菌酶水平，替代溶血补体活性（ACH（50））和抗体滴度高于单独用该菌苗免疫的鱼。但是，两组之间的差异并不总是一致的。在用佐剂制剂免疫的鱼中，免疫后长达12周的相对存活率（RPS）达到85-100％，而仅用细菌素免疫的鱼的相对存活率在75-90％的范围内。我们的数据表明，Montanide ISA 763 AVG用作可注射的Y. ruckeri疫苗的佐剂可以提高虹鳟鱼的存活率和免疫应答。

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