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首页> 外文期刊>Genes to cells : >Transcription-mediated hyper-recombination in HOT1.
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Transcription-mediated hyper-recombination in HOT1.

机译：HOT1中转录介导的超重组。

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摘要

Recombination hotspots are DNA sequences which enhance recombination around that region. HOT1 is one of the best-studied mitotic hotspots in yeast. HOT1 includes a RNA polymerase I (PolI) transcription promoter which is responsible for 35S ribosomal rRNA gene (rDNA) transcription. In a PolI defective mutant the HOT1 hotspot activity is abolished, therefore transcription of HOT1 is thought to be an important factor for the recombination stimulation. However, it is not clear whether the transcription itself or other pleiotropic phenotypes stimulates recombination. To investigate the role of transcription, we made a highly activated PolI transcription system in HOT1 by using a strain whose rDNA repeats are deleted (rdnDeltaDelta). In the rdnDeltaDelta strain, HOT1 transcription was increased about 14 times compared to wild-type. Recombination activity stimulated by HOT1 in this strain was also elevated, about 15 times, compared to wild-type. These results indicate that the level of PolI transcription in HOT1 determines efficiency of the recombination. Moreover, Fob1p, which is essential for both the recombination stimulation activity and transcription of HOT1, was dispensable in the rdnDeltaDelta strains. This suggests that Fob1p is functioning as a PolI transcriptional activator in the wild-type strain.
机译：重组热点是增强该区域周围重组的DNA序列。 HOT1是酵母中研究最深入的有丝分裂热点之一。 HOT1包含一个RNA聚合酶I（PolI）转录启动子，负责35S核糖体rRNA基因（rDNA）转录。在PolI缺陷型突变体中，HOT1热点活性被取消，因此，HOT1的转录被认为是重组刺激的重要因素。但是，尚不清楚转录本身或其他多效性表型是否会刺激重组。为了研究转录的作用，我们使用了缺失rDNA重复序列的菌株（rdnDeltaDelta）在HOT1中建立了高度激活的PolI转录系统。在rdnDeltaDelta菌株中，与野生型相比，HOT1转录增加了约14倍。与野生型相比，HOT1在该菌株中刺激的重组活性也提高了约15倍。这些结果表明，HOT1中PolI转录的水平决定了重组效率。而且，对于重组刺激活性和HOT1的转录都至关重要的Fob1p在rdnDeltaDelta菌株中是必需的。这表明Fob1p在野生型菌株中起着PolI转录激活剂的作用。

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