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首页> 外文期刊>Genes to cells : >Initiator titration complex formed at datA with the aid of IHF regulates replication timing in Escherichia coli (vol 14, pg 329, 2009)
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Initiator titration complex formed at datA with the aid of IHF regulates replication timing in Escherichia coli (vol 14, pg 329, 2009)

机译：在datA上借助IHF形成的引发剂滴定复合物可调节大肠杆菌中的复制时机（第14卷，第329页，2009年）

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摘要

DnaA is a replication initiator protein that is conserved among bacteria. It plays a central role in the initiation of DNA replication. In order to monitor its behavior in living Escherichia coli cells, a nonessential portion of the protein was replaced by a fluorescent protein. Such a strain grew normally, and flow cytometry data suggested that the chimeric protein has no substantial loss of the initiator activity. The initiator was distributed all over the nucleoid. Furthermore, a majority of the cells exhibited certain distinct foci that emitted bright fluorescence. These foci colocalized with the replication origin (oriC) region and were brightest during the period spanning the initiation event. In cells that had undergone the initiation, the foci were enriched in less intense ones. In addition, a significant portion of the oriC regions at this cell cycle stage had no colocalized DnaA-enhanced yellow fluorescent protein (EYFP) focus point.
机译：DnaA是在细菌之间保守的复制起始蛋白。它在DNA复制的启动中起着核心作用。为了监测其在活的大肠杆菌细胞中的行为，该蛋白质的非必需部分被荧光蛋白质替代。这种菌株正常生长，流式细胞仪数据表明该嵌合蛋白没有实质性的引发剂活性损失。引发剂分布在整个核苷上。此外，大多数细胞表现出某些不同的病灶，这些病灶发出明亮的荧光。这些病灶与复制起点（oriC）区共定位，并且在整个启动事件期间最亮。在经历启动的细胞中，病灶富含强度较弱的病灶。此外，在此细胞周期阶段的大部分oriC区域没有共定位的DnaA增强的黄色荧光蛋白（EYFP）焦点。

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