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首页> 外文期刊>Genes to cells : >BTB protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex
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BTB protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex

机译：BTB蛋白dKLHL18 / CG3571充当dCul3泛素连接酶复合物的衔接子亚基

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摘要

The BTB domain is a highly conserved protein-protein interaction motif and functions in diverse cellular processes, including transcriptional regulation, ion channel assembly, cytoskeleton dynamics and apoptosis. Recently, it was reported that some BTB domain-containing proteins associate with Cullin-3 (Cul3), an E3 ubiquitin ligase, and act as an adaptor for Cul3 recognition of its substrate. However, the target substrates for the Cul3/BTB protein E3 ubiquitin ligase complex are largely unknown. Here, we report the characterization of a novel Drosophila BTB protein, dKLHL18/CG3571. By purification of a dKLHL18-associated complex, we identified CG10324, CG5808, l(2)37Cb and dCul3/guftagu. Indeed, the physical association of dKLHL18 with these proteins was observed in insect S2 cells, and genetic interactions among the identified factors were also observed in thorax development. Moreover, transient overexpression of dKLHL18 increased the ubiquitinated protein levels of CG10324 and CG5808. These findings suggest that dKLHL18 is an adaptor for a dCul3 E3 ubiquitin ligase to accommodate CG10324, CG5808 and l(2)37Cb proteins for ubiquitination.
机译：BTB结构域是高度保守的蛋白质-蛋白质相互作用基序，并在多种细胞过程中起作用，包括转录调控，离子通道组装，细胞骨架动力学和细胞凋亡。最近，据报道，一些含BTB结构域的蛋白质与E3泛素连接酶Cullin-3（Cul3）结合，并充当Cul3识别其底物的衔接子。但是，Cul3 / BTB蛋白E3泛素连接酶复合物的靶底物在很大程度上是未知的。在这里，我们报告一种新型果蝇BTB蛋白dKLHL18 / CG3571的表征。通过纯化与dKLHL18相关的复合物，我们鉴定出CG10324，CG5808、1（2）37Cb和dCul3 / guftagu。确实，在昆虫S2细胞中观察到了dKLHL18与这些蛋白质的物理联系，并且在胸部发育中也观察到了已鉴定因子之间的遗传相互作用。此外，dKLHL18的瞬时过表达增加了CG10324和CG5808的泛素化蛋白水平。这些发现表明，dKLHL18是dCul3 E3泛素连接酶的衔接子，可容纳CG10324，CG5808和1（2）37Cb蛋白用于泛素化。

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