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首页> 外文期刊>Genome >A high-resolution, intraspecific linkage map of pepper (Capsicum annuum L.) and selection of reduced recombinant inbred line subsets for fast mapping
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A high-resolution, intraspecific linkage map of pepper (Capsicum annuum L.) and selection of reduced recombinant inbred line subsets for fast mapping

机译：辣椒的高分辨率，种内连锁图谱（Capsicum annuum L.）和减少重组的近交系亚群的选择，以便快速作图

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A high-resolution, intraspecific linkage map of pepper (Capsicum annuum L.) was constructed from a population of 297 recombinant inbred lines. The parents were the large-fruited inbred cultivar `Yolo Wonder' and the hot pepper line `Criollo de Morelos 334', which is heavily used as a source of resistance to a number of diseases. A set of 587 markers (507 amplified fragment length polymorphisms, 40 simple sequence repeats, 19 restriction fragment length polymorphisms, 17 sequence-specific amplified polymorphisms, and 4 sequence tagged sites) were used to generate the map; of these, 489 were assembled into 49 linkage groups (LGs), including 14 LGs with 10 to 60 markers per LG and 35 with 2 to 9 markers per LG. The framework map covered 1857 cM with an average intermarker distance of 5.71 cM. Twentythree LGs, composed of 69% of the markers and covering 1553 cM, were assigned to l of the 12 haploid pepper chromosomes, leaving 26 LGs (304 cM) unassigned. The chromosome framework map built with 250 markers led to a high level of mapping confidence and an average intermarker distance of 6.54 cM. By applying MapPop software, it was possible to select smaller subsets of 141 or 93 most informative individuals with a view to reducing the time and cost of further mapping and phenotyping. To define the smallest number of individuals sufficient for assigning any new marker to a chromosome, subsets from 12 to 45 individuals and a set of 13 markers distributed over all 12 chromosomes were screened. In most cases, the markers were correctly assigned to their expected chromosome, but the accuracy of the map position decreased as the number of individuals was reduced.
机译：从297个重组自交系群体中构建了辣椒的高分辨率，种内连锁图谱（Capsicum annuum L.）。父母是大果的近交栽培品种“ Yolo Wonder”和辣椒品种“ Criollo de Morelos 334”，其被广泛用作抵抗多种疾病的来源。使用一组587个标记（507个扩增片段长度多态性，40个简单序列重复，19个限制性片段长度多态性，17个序列特异性扩增多态性和4个序列标记位点）生成图谱;其中，489个被组装成49个连锁组（LG），包括14个LG，每个LG 10至60个标记，35个LG，每个LG 2至9个标记。框架图覆盖1857 cM，平均标记间距离为5.71 cM。将由69％的标记组成并覆盖1553 cM的23个LG分配给12个单倍体胡椒染色体中的1个，剩下26个LG（304 cM）未分配。用250个标记构建的染色体框架图导致较高的图谱置信度和6.54 cM的平均标记间距离。通过应用MapPop软件，可以选择141个或93个最有信息的个体的较小子集，以减少进一步作图和表型划分的时间和成本。为了定义足以将任何新标记分配给染色体的最少数量的个体，筛选了12至45个个体的子集以及分布在所有12条染色体上的13个标记的集合。在大多数情况下，标记已正确分配给它们的预期染色体，但是随着个体数量的减少，地图位置的准确性降低了。

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