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Genome size and genome evolution in diploid Triticeae species

机译:二倍体小麦的基因组大小和基因组进化

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One of the intriguing issues concerning the dynamics of plant genomes is the occurrence of intraspecific variation in nuclear DNA amount. The aim of this work was to assess the ranges of intraspecific, interspecific, and intergeneric variation in nuclear DNA content of diploid species of the tribe Triticeae (Poaceae) and to examine the relation between life form or habitat and genome size. Altogether, 438 plants representing 272 lines that belong to 22 species were analyzed. Nuclear DNA content was estimated by flow cytometry. Very small intraspecific variation in DNA amount was found between lines of Triticeae diploid species collected from different habitats or between different morphs. In contrast to the constancy in nuclear DNA amount at the intraspecific level, there are significant differences in genome size between the various diploid species. Within the genus Aegilops, the 1C DNA amount ranged from 4.84 pg in A. caudata to 7.52 pg in A. sharonensis; among genera, the I C DNA amount ranged from 4.18 pg in Heteranthelium piliferum to 9.45 pg in Secale montanum. No evidence was found for a smaller genome size in annual, self-pollinating species relative to perennial, cross-pollinating ones. Diploids that grow in the southern part of the group's distribution have larger genomes than those growing in other parts of the distribution. The contrast between the low variation at the intraspecific level and the high variation at the interspecific one suggests that changes in genome size originated in close temporal proximity to the speciation event, i.e., before, during, or immediately after it. The possible effects of sudden changes in genome size on speciation processes are discussed.
机译:与植物基因组动力学有关的有趣问题之一是核内DNA量发生种内变异的情况。这项工作的目的是评估Triticeae(禾本科)二倍体物种的核DNA含量的种内,种间和种间变异范围,并研究生命形式或栖息地与基因组大小之间的关系。总共分析了代表22个属的272个品系的438株植物。通过流式细胞术估计核DNA含量。发现从不同生境或不同形态之间收集到的小麦二倍体种系之间的DNA量存在非常小的种内变异。与种内水平的核DNA量恒定相比,各种二倍体物种之间的基因组大小存在显着差异。在Aegilops属中,1C DNA的含量范围从A. caudata中的4.84 pg到A. sharonensis中的7.52 pg。在各属中,I C DNA的量范围从皮脂杂种中的4.18 pg到Secale montanum中的9.45 pg。没有证据表明一年生自花传粉物种的基因组大小小于多年生异花传粉物种。在该组分布的南部生长的二倍体具有比在该分布的其他部分生长的二倍体更大的基因组。种内水平的低变化与种间水平的高变化之间的对比表明,基因组大小的变化起源于物种形成事件的时间附近,即物种形成之前,期间或之后。讨论了基因组大小突然变化对物种形成过程的可能影响。

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