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首页> 外文期刊>Genome >Genome-wide identification and expression analysis of the apple ASR gene family in response to Alternaria alternata f. sp mali
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Genome-wide identification and expression analysis of the apple ASR gene family in response to Alternaria alternata f. sp mali

机译：苹果ASR基因家族响应链格孢的全基因组鉴定和表达分析。马里

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摘要

The ABA/water stress/ripening-induced (ASR) gene family exists universally in higher plants, and many ASR genes are up-regulated during periods of environmental stress and fruit ripening. Although a considerable amount of research has been performed investigating ASR gene response to abiotic stresses, relatively little is known about their roles in response to biotic stresses. In this report, we identified five ASR genes in apple (Malus x domestica) and explored their phylogenetic relationship, duplication events, and selective pressure. Five apple ASR genes (Md-ASR) were divided into two clades based on phylogenetic analysis. Species-specific duplication was detected in M. domestica ASR genes. Leaves of 'Golden delicious' and 'Starking' were infected with Alternaria alternata f. sp. mali, which causes apple blotch disease, and examined for the expression of the ASR genes in lesion areas during the first 72 h after inoculation. Md-ASR genes showed different expression patterns at different sampling times in 'Golden delicious' and 'Starking'. The activities of stress-related enzymes, peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), phenylalanine ammonia lyase (PAL), and polyphenoloxidase (PPO), and the content of malondialdehyde (MDA) were also measured in different stages of disease development in two cultivars. The ASR gene expression patterns and theses physiological indexes for disease resistance suggested that Md-ASR genes are involved in biotic stress responses in apple.
机译：ABA /水分胁迫/成熟诱导（ASR）基因家族普遍存在于高等植物中，许多ASR基因在环境胁迫和果实成熟期间被上调。尽管已经进行了大量研究以研究ASR基因对非生物胁迫的反应，但关于它们对生物胁迫的作用的了解相对较少。在本报告中，我们鉴定了苹果（Malus x domestica）中的五个ASR基因，并探讨了它们的系统发生关系，复制事件和选择压力。根据系统发育分析，将五个苹果ASR基因（Md-ASR）分为两个进化枝。在M. domestica ASR基因中检测到物种特异性重复。用金黄链霉菌（Alternaria alternata f）感染“黄金美味”和“斯塔克星”的叶片。 sp。引起苹果斑点病的马里，在接种后的头72小时内检查病灶区域中ASR基因的表达。 Md-ASR基因在“金黄色的美味”和“斯塔克的”中在不同的采样时间显示出不同的表达模式。还测量了应激相关酶，过氧化物酶（POD），超氧化物歧化酶（SOD），过氧化氢酶（CAT），苯丙氨酸氨裂合酶（PAL）和多酚氧化酶（PPO）的活性以及丙二醛（MDA）的含量两个品种的疾病发展阶段。 ASR基因的表达模式和这些抗病生理指标表明，Md-ASR基因参与了苹果的生物胁迫反应。

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