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Microsatellite-based genetic relationships in thegenus Camellia: potential for improving cultivars

机译：茶花属中基于微卫星的遗传关系：改良品种的潜力

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摘要

There is a lack of published microsatellite data that characterizes Camellia spp. To address this, an initial study of sequence tagged microsatellite site (STMS) variation was undertaken with 132 accessions of Camellia spp., which in-cluded 24 accessions representing 22 different species or varieties as well as 63 cultivars of C. japonica, 33 cultivars of C. sasanqua, 7 cultivars of C. xvernalis, 3 cultivars of C. xhiemalis, and 2 cultivars of C. hybrida. The four primer sets used (MSCJAF37, MSCJAH46, MSCJAF25, and MSCJAH38) successfully amplified polymorphic alleles in all the species analysed, showing cross-transferability. Overall, 96 alleles were scored. MSCJAH38 primers produced the highest number of bands (30), while MSCJAH46 primers yielded the lowest number (15). The genetic distance between pairs of accessions was estimated on the basis of the Nei coefficient and a principal coordinate analysis was performed. The plot revealed a main differentiation between the C. japonica cultivars and the winter camellias. The distribution of the genetic variation, attributed by AMOVA, particularly highlighted genetic overlap among C. sasanqua cultivars and the cultivars belonging to C. xvernalis, C. xhiemalis, and C. hybrida. In conclusion, this study demonstrated that STMS markers offer a suitable method for detection of genetic variability and molecular study of camellia genotypes.
机译：缺乏表征茶花属的微卫星数据。为了解决这个问题，对序列标记的微卫星位点（STMS）变异进行了初步研究，其中有132个山茶种，其中包括代表22个不同物种或品种的24个种以及63个日本粳稻，33个品种。 Sasanqua，7个C. xvernalis品种，3个C. xhiemalis和2个C. hybrida品种。使用的四个引物组（MSCJAF37，MSCJAH46，MSCJAF25和MSCJAH38）成功扩增了所有分析物种中的多态性等位基因，表现出交叉转移性。总体上，对96个等位基因进行了评分。 MSCJAH38引物产生的条带数量最多（30），而MSCJAH46引物产生的条带数量最少（15）。基于Nei系数估计成对的种质间的遗传距离，并进行主坐标分析。该图显示了日本粳稻品种和冬季山茶花之间的主要区别。由AMOVA归因于遗传变异的分布，尤其突出了茶蜡树栽培种与属于角蜡状梭菌，角质蜡化菌和杂交角质梭菌的品种之间的遗传重叠。总之，这项研究表明，STMS标记为山茶基因型的遗传变异性检测和分子研究提供了一种合适的方法。

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