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首页> 外文期刊>Genome >Characterization and mapping of a male-sterility mutant, tapetum desquamation (t), in rice.
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Characterization and mapping of a male-sterility mutant, tapetum desquamation (t), in rice.

机译：水稻中雄性不育突变体绒毡层脱皮（t）的表征和作图。

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A spontaneously mutated male-sterile material was found among the offspring of the indica restorer line Jinhuiyihao. To understand the status and function of the related gene and clone the gene, a near-isogenic line (NIL) of the male sterility was bred, and characterization of the mutant and gene mapping were performed. The results indicated that there are obvious differences between the male-sterile NIL and the indica maintainer line II-32B. The anther size of the NIL is smaller than that of II-32B, and the anther color is white in the NIL but yellow in II-32B. No pollen from the matured anther in the NIL was observed to be stained using KI-I2 solution. In transverse sections of the sterile anther, at early microspore stage the cytoplasm of the tapetum concentrates but the tapetum itself does not degenerate after microspores are released from the tetrads; the tapetum then desquamates from the anther wall and enwraps microspores; subsequently, the surrounded microspores collapse completely at late microspore and early bicellular pollen stages. Inheritance analysis showed that the male sterility was controlled by a single recessive gene, ostd (t). This gene was mapped between the SSR markers RM7434 and RM275 on chromosome 6, and the physical distance from RM7434 to RM275 is about 389 kb.
机译：在the恢复系金慧一号的后代中发现了自发突变的雄性不育材料。为了了解相关基因的状态和功能并克隆该基因，繁殖了雄性不育的近等基因系（NIL），并进行了突变体鉴定和基因定位。结果表明，雄性不育的NIL和the型保持系II-32B之间存在明显差异。 NIL的花药尺寸小于II-32B，而NIL的花药颜色为白色，而II-32B的花药颜色为黄色。使用KI-1溶液未观察到NIL中来自成熟花药的花粉被染色。在无菌花药的横切面中，在小孢子早期，绒毡层的细胞质浓缩，但是在从四分体中释放出微孢子后绒毡层本身并不会退化。然后绒毡层从花药壁上脱落并包裹小孢子。随后，包围的小孢子在小孢子晚期和早期双细胞花粉阶段完全崩溃。遗传分析表明，雄性不育由一个隐性基因ostd（t）控制。该基因被定位在6号染色体上的SSR标记RM7434和RM275之间，从RM7434到RM275的物理距离约为389 kb。

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