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首页> 外文期刊>Genes, Chromosomes and Cancer >Contiguous arrangement of p45 NFE2, HnRNP A1, and HP1 alpha on mouse chromosome 15 and human chromosome 12: evidence for suppression of these genes due to retroviral integration within the Fli-2 locus.
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Contiguous arrangement of p45 NFE2, HnRNP A1, and HP1 alpha on mouse chromosome 15 and human chromosome 12: evidence for suppression of these genes due to retroviral integration within the Fli-2 locus.

机译：p45 NFE2，HnRNP A1和HP1 alpha在小鼠15号染色体和人12号染色体上的连续排列：由于Fli-2基因座内的逆转录病毒整合，这些基因受到抑制的证据。

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摘要

Fli-2 is a common site of proviral integration in multistage erythroleukemia cells induced by Friend murine leukemia virus (F-MuLV) or the polycythemia strain of Friend leukemia virus (FV-P). Previously, we reported that integration of Friend virus into the Fli-2 locus in CB3, an erythroleukemia cell line that harbors a homozygous inactivation of the Fli-2 locus, results in the loss of expression of two genes encoding the 45-kDa subunit of the erythroid-specific nuclear factor p45 NFE2 and the splicing factor HnRNP A1. Here, we report the identification of a third gene, Heterochromatin protein 1 (HP1alpha, also known as CBX5), which is located downstream of HnRNP A1, and p45 NFE2. Northern blot analysis revealed that the expression of HP1alpha, along with p45 NFE2 and HnRNP A1, is either undetectable or substantially reduced in CB3 cells, suggesting that HP1alpha expression is also regulated by proviral insertion within the Fli-2 locus in CB3 cells. Because p45 NFE2 was previously mapped to mouse chromosome 15, our results demonstrate that HP1alpha and HnRNP A1 are also located on mouse chromosome 15 and that the p45 NFE2, HnRNP A1, and HP1alpha genes are arranged contiguously. Contiguous arrangement of these three genes was also detected in man; this consequently localizes HP1alpha to human chromosome band 12q13.
机译：Fli-2是Friend鼠白血病病毒（F-MuLV）或Friend白血病病毒的多性红细胞株（FV-P）诱导的多阶段红白血病细胞中前病毒整合的常见位点。之前，我们曾报道Friend病毒整合到CB3（具有Fli-2基因座纯合失活的红白血病细胞系）CB3的Fli-2基因座中，导致两个编码该基因45 kDa亚基的基因丧失表达红系特异性核因子p45 NFE2和剪接因子HnRNP A1。在这里，我们报告鉴定的第三个基因，异染色质蛋白1（HP1alpha，也称为CBX5），位于HnRNP A1和p45 NFE2的下游。 Northern印迹分析显示HP1alpha以及p45 NFE2和HnRNP A1的表达在CB3细胞中无法检测或显着降低，这表明HP1alpha的表达也受CB3细胞Fli-2基因座中前病毒插入的调节。因为p45 NFE2先前已映射到小鼠15号染色体，所以我们的结果证明HP1alpha和HnRNP A1也位于小鼠15号染色体上，并且p45 NFE2，HnRNP A1和HP1alpha基因是连续排列的。在人类中也检测到这三个基因的连续排列。因此，这将HP1alpha定位于人类染色体带12q13。

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