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首页> 外文期刊>Biochemistry >Solution structure of Ace-AMP1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins
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Solution structure of Ace-AMP1, a potent antimicrobial protein extracted from onion seeds. Structural analogies with plant nonspecific lipid transfer proteins

机译：从洋葱种子中提取的有效抗菌蛋白Ace-AMP1的溶液结构。与植物非特异性脂质转移蛋白的结构类比

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The three-dimensional solution structure of Ace-AMP1, an antifungal protein extracted from onion seeds, was determined using H-1 NMR spectroscopy and molecular modeling, This cationic protein contains 93 amino acid residues and four disulfide bridges, Its structure was determined from 1260 NOE-derived distance restraints and 173 dihedral restraints derived from NOEs and (3)J(C2HNH) coupling constants. The global fold involves four helical segments connected by three loops and a C-terminal tail without regular secondary structures, except for a 3(10)-helix turn and a beta-turn. The most striking feature is the absence of any continuous cavity running through the whole molecule as found in recently determined structures of nonspecific transfer proteins extracted from wheal and maize seeds, although their global folds are very similar. Consistent with the absence of a cavity in the core of Ace-AMP1, it was found that this protein, in contrast to ns-LTPs, does not bind fluorescently labeled phospholipids in solution. On the other hand, Ace-AMP1 is able to interact with phospholipid membranes as shown by the release of carboxyfluorescein from the lumen of artificial liposomes and by the induction of alterations in fluorescence polarization of fluorescently labeled phospholipids embedded in artificial liposomes. [References: 69]
机译：使用H-1 NMR光谱和分子建模确定了从洋葱种子提取的抗真菌蛋白Ace-AMP1的三维溶液结构，该阳离子蛋白包含93个氨基酸残基和四个二硫键，其结构由1260年确定从NOE和（3）J（C2HNH）耦合常数得出NOE派生的距离约束和173个二面约束。全局折叠包括通过三个环和一个C末端的尾部相连的四个螺旋段，这些尾部没有规则的二级结构，除了3（10）螺旋圈和一个beta圈。最显着的特征是，从最近发现的从鲸鱼和玉米种子中提取的非特异性转移蛋白的结构中发现，没有贯穿整个分子的连续空腔，尽管它们的整体折叠非常相似。与Ace-AMP1的核心中没有空腔相一致，发现与ns-LTP相比，该蛋白质在溶液中不结合荧光标记的磷脂。另一方面，Ace-AMP1能够与磷脂膜相互作用，这是通过从人工脂质体腔释放羧基荧光素，以及通过诱导嵌入人工脂质体中的荧光标记磷脂的荧光偏振改变而显示的。 [参考：69]

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