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首页> 外文期刊>Genomics >Molecular cloning, functional characterization, tissue distribution, and chromosomal localization of a human, small intestinal sodium-phosphate (Na+-Pi) transporter (SLC34A2).
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Molecular cloning, functional characterization, tissue distribution, and chromosomal localization of a human, small intestinal sodium-phosphate (Na+-Pi) transporter (SLC34A2).

机译：人类小肠磷酸钠（Na + -Pi）转运蛋白（SLC34A2）的分子克隆，功能表征，组织分布和染色体定位。

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摘要

Phosphate plays a crucial role in cellular metabolism, and its homeostatic regulation in intestinal and renal epithelia is critical. Apically expressed sodium-phosphate (Na(+)-P(i)) transporters play a critical role in this regulation. We have isolated a cDNA (HGMW-approved symbol SLC34A2) encoding a novel human small intestinal Na(+)-P(i) transporter. The cDNA is shown to be 4135 bp in length with an open reading frame that predicts a 689-amino-acid polypeptide. The putative protein has 76% homology to mouse intestinal type II Na(+)-P(i) transporter (Na/Pi-IIb) and lower homologies with renal type II Na(+)-P(i) transporters. Northern blots showed a singular transcript of 5.0 kb in human lung, small intestine, and kidney. Computer analysis suggests a protein with 11 transmembrane domains and several potential posttranslational modification sites. Functional characterization in Xenopus laevis oocytes showed that this cDNA encodes a functional Na(+)-P(i) transporter. Furthermore, the gene encoding this cDNA was mapped to human chromosome 4p15.1-p15.3 by the FISH method. Copyright 1999 Academic Press.
机译：磷酸盐在细胞代谢中起着至关重要的作用，其在肠和肾上皮细胞中的稳态调节至关重要。顶端表达的磷酸钠（Na（+）-P（i））转运蛋白在该法规中起关键作用。我们已经分离出编码新型人类小肠Na（+）-P（i）转运蛋白的cDNA（HGMW批准的符号SLC34A2）。显示该cDNA的长度为4135 bp，带有一个开放阅读框，该框预测了一个689个氨基酸的多肽。推定的蛋白质与小鼠肠道II型Na（+）-P（i）转运蛋白（Na / Pi-IIb）具有76％的同源性，与肾脏II型Na（+）-P（i）转运蛋白具有较低的同源性。 Northern印迹显示在人的肺，小肠和肾脏中有一个5.0 kb的奇异转录本。计算机分析表明该蛋白具有11个跨膜结构域和几个潜在的翻译后修饰位点。非洲爪蟾卵母细胞的功能表征表明该cDNA编码功能性Na（+）-P（i）转运蛋白。此外，通过FISH方法将编码该cDNA的基因定位于人染色体4p15.1-p15.3。版权所有1999 Academic Press。

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