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首页> 外文期刊>Genomics >Characterization of mRAD18Sc, a mouse homolog of the yeast postreplication repair gene RAD18.
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Characterization of mRAD18Sc, a mouse homolog of the yeast postreplication repair gene RAD18.

机译：mRAD18Sc的特征，酵母复制后修复基因RAD18的小鼠同源物。

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摘要

The RAD18 gene of the yeast Saccharomyces cerevisiae encodes a protein with ssDNA binding activity that interacts with the ubiquitin-conjugating enzyme RAD6 and plays an important role in postreplication repair. We identified and characterized the putative mouse homolog of RAD18, designated mRAD18Sc. The mRAD18Sc open reading frame encodes a 509-amino-acid polypeptide that is strongly conserved in size and sequence between yeast and mammals, with specific conservation of the RING-zinc-finger and the classic zinc-finger domain. The degree of sequence conservation between mRAD18Sc, RAD18, and homologous sequences identified in other species (NuvA from Aspergillus nidulans and Uvs-2 from Neurospora crassa) is entirely consistent with the evolutionary relationship of these organisms, strongly arguing that these genes are one another's homologs. Consistent with the presence of a nuclear translocation signal in the amino acid sequence, we observed the nuclear localization of GFP-tagged mRAD18Sc after stable transfection to HeLa cells. mRNA expression of mRAD18Sc in the mouse was observed in thymus, spleen, brain, and ovary, but was most pronounced in testis, with the highest level of expression in pachytene-stage primary spermatocytes, suggesting that mRAD18Sc plays a role in meiosis of spermatogenesis. Finally, we mapped the mRAD18Sc gene on mouse chromosome 6F. Copyright 2000 Academic Press.
机译：酵母酿酒酵母的RAD18基因编码具有ssDNA结合活性的蛋白，该蛋白与泛素结合酶RAD6相互作用并在复制后修复中起重要作用。我们鉴定并鉴定了RAD18的推定小鼠同源物，称为mRAD18Sc。 mRAD18Sc开放阅读框编码一个509个氨基酸的多肽，在酵母和哺乳动物之间的大小和序列高度保守，并具有RING-锌指和经典锌指结构域的特异性保守性。 mRAD18Sc，RAD18和其他物种（nidulans中的NuvA和crus Neurospora crassa中的Uvs-2）鉴定到的同源序列之间的序列保守程度与这些生物的进化关系完全一致，强烈认为这些基因是彼此的同源物。 。与氨基酸序列中核易位信号的存在一致，我们观察到稳定转染至HeLa细胞后GFP标记的mRAD18Sc的核定位。在小鼠的胸腺，脾脏，大脑和卵巢中观察到mRAD18Sc的mRNA表达，但在睾丸中最为明显，在粗线期初生精母细胞中表达水平最高，表明mRAD18Sc在精子发生减数分裂中起作用。最后，我们将mRAD18Sc基因定位在小鼠6F染色体上。版权所有2000学术出版社。

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