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Locked nucleic acids in PCR primers increase sensitivity and performance.

机译：PCR引物中的锁定核酸可提高灵敏度和性能。

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摘要

The incorporation of locked nucleic acids (LNAs) into oligonucleotide primers has been shown to increase template binding strength and specificity for DNA amplification. Real-time PCR and DNA sequencing have been shown to be significantly enhanced by the use of LNAs. Theoretically, increasing primers' binding strength may also increase the sensitivity of conventional PCR, reducing minimum template requirements. We compared LNA-modified PCR primers with their standard DNA counterparts for amplification sensitivity with template amounts as low as 5 pg. Although the results are highly dependent on the design of the LNA primers, large increases in peak height can be achieved from as little as 75 pg, as well as clearer and more complete profiles. Increased amplification success with lower template amounts may also be seen. Additionally, the use of LNAs can enhance multiplexing. Thus, incorporating LNAs into PCR primers can increase amplification success, sensitivity, and performance under a wide range of conditions.
机译：已显示将锁定的核酸（LNA）掺入寡核苷酸引物中会增加模板结合强度和DNA扩增的特异性。已经显示，通过使用LNA，实时PCR和DNA测序显着增强。从理论上讲，增加引物的结合强度还可以提高常规PCR的灵敏度，从而降低最低模板要求。我们将LNA修饰的PCR引物与标准DNA对应物进行了比较，其模板的扩增灵敏度低至5 pg。尽管结果高度依赖于LNA引物的设计，但峰高可以从低至75 pg以及更大，更清晰，更完整的轮廓中实现。还可以看到以较低的模板量增加的扩增成功率。此外，使用LNA可以增强多路复用。因此，将LNA掺入PCR引物中可在多种条件下提高扩增成功率，灵敏度和性能。

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