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First complete chromosomal organization of a protozoan plant parasite (Phytomonas spp.).

机译:原生动物寄生虫(Phytomonas spp。)的第一个完整的染色体组织。

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摘要

Phytomonas spp. are members of the family Trypanosomatidae that parasitize plants and may cause lethal diseases in crops such as Coffee Phloem necrosis, Hartrot in coconut, and Marchitez sorpresiva in oil palm. In this study, the molecular karyotype of 6 isolates from latex plants has been entirely elucidated by pulsed-field gel electrophoresis and DNA hybridization. Twenty-one chromosomal linkage groups constituting heterologous chromosomes and sizing between 0.3 and 3 Mb could be physically defined by the use of 75 DNA markers (sequence-tagged sites and genes). From these data, the genome size can be estimated at 25.5 (+/-2) Mb. The physical linkage groups were consistently conserved in all strains examined. Moreover, the finding of several pairs of different-sized homologous chromosomes strongly suggest diploidy for this organism. The definition of the complete molecular karyotype of Phytomonas represents an essential primary step toward sequencing the genome of this parasite of economical importance.
机译:假单胞菌是Trypanosomatidae科的成员,它寄生植物,并可能导致作物致死性疾病,如咖啡韧皮部坏死,椰子中的哈特罗特和油棕中的马尔基什松皮。在这项研究中,已通过脉冲场凝胶电泳和DNA杂交完全阐明了来自乳胶植物的6种分离物的分子核型。通过使用75个DNA标记(序列标记的位点和基因),可以物理定义构成异源染色体且大小在0.3和3 Mb之间的21个染色体连接基团。根据这些数据,基因组大小可以估计为25.5(+/- 2)Mb。物理连锁组在所有检查的菌株中均保持保守。而且,发现几对不同大小的同源染色体强烈提示了该生物的二倍体。番茄疫霉菌的完整分子核型的定义代表了对该具有经济重要性的寄生虫的基因组进行测序的必不可少的第一步。

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