...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >Microsatellite instability, promoter methylation and protein expression of the DNA mismatch repair genes in epithelial ovarian cancer
【24h】

Microsatellite instability, promoter methylation and protein expression of the DNA mismatch repair genes in epithelial ovarian cancer

机译：上皮性卵巢癌微卫星不稳定性，启动子甲基化和DNA错配修复基因的蛋白表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The role of defective mismatch repair (MMR) system in ovarian carcinoma is not well defined. The purpose of the study was to determine the relationship between microsatellite instability (MSI), promoter methylation and protein expression of MMR genes in epithelial ovarian carcinoma (EOC). MSI and promoter methylation of MLH1, MSH2 and PMS2 genes were studied using PCR methods in the study cohort. A small subset of samples was used to analyze the protein expression by immunohistochemistry (IHC). MSI was observed in >60% of tumor samples and 47% of normal ovaries. MLH1 was methylated in 37.5% and 64.3% EOCs and LMP tumors. The loss of immunoexpression of MMR genes was not seen in ovarian tumors. There was no correlation between MSI, promoter methylation and protein expression of the MMR genes suggesting that each may function independently. MSI is a common event in ovarian carcinoma and may increase the clinical awareness of the subset of tumors. (C) 2014 Elsevier Inc. All rights reserved.
机译：有缺陷的错配修复（MMR）系统在卵巢癌中的作用尚不明确。这项研究的目的是确定上皮性卵巢癌（EOC）中的微卫星不稳定性（MSI），启动子甲基化和MMR基因蛋白表达之间的关系。在研究队列中使用PCR方法研究了MLH1，MSH2和PMS2基因的MSI和启动子甲基化。一小部分样品用于通过免疫组织化学（IHC）分析蛋白质表达。在60％以上的肿瘤样本和47％的正常卵巢中观察到MSI。 MLH1在37.5％和64.3％的EOC和LMP肿瘤中被甲基化。在卵巢肿瘤中未见MMR基因免疫表达的丧失。 MSI，MMR基因的启动子甲基化和蛋白表达之间没有相关性，表明它们可能各自独立起作用。 MSI是卵巢癌中的常见事件，可能会增加对肿瘤子集的临床认识。 （C）2014 Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号