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首页> 外文期刊>Genomics >A BAC clone-based physical map of ovine major histocompatibility complex.
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A BAC clone-based physical map of ovine major histocompatibility complex.

机译：基于BAC克隆的绵羊主要组织相容性复合体的物理图谱。

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摘要

An ovine bacterial artificial chromosome (BAC) library containing 190,000 BAC clones was constructed and subsequently screened to construct a BAC-based physical map for the ovine major histocompatibility complex (MHC). Two hundred thirty-three BAC clones were selected by 84 overgo probes designed on human, mouse, and swine MHC sequence homologies. Ninety-four clones were ordered by DNA fingerprinting to form contigs I, II, and III that correspond to ovine MHC class I-class III, class IIa, and class IIb. The minimum tiling paths of contigs I, II, and III are 15, 4, and 4 BAC clones, spanning approximately 1900, 400, and 300 kb, respectively. The order and orientation of most BAC clones in each contig were confirmed by BAC-end sequencing. An open gap exists between class IIa and class III. This work helps to provide a foundation for detailed study of ovine MHC genes and of evolution of MHCs in mammals.
机译：构建了包含190,000个BAC克隆的绵羊细菌人工染色体（BAC）文库，随后对其进行筛选以构建基于BAC的绵羊主要组织相容性复合体（MHC）物理图谱。通过在人，小鼠和猪MHC序列同源性上设计的84种过度探针选择了233个BAC克隆。通过DNA指纹图谱对94个克隆进行排序，以形成对应于绵羊MHC I类，III类，IIa类和IIb类的重叠群I，II和III。重叠群I，II和III的最小平铺路径是15、4和4个BAC克隆，分别跨越大约1900、400和300 kb。每个重叠群中大多数BAC克隆的顺序和方向已通过BAC末端测序确认。 IIa级和III级之间存在开放的鸿沟。这项工作有助于为详细研究绵羊MHC基因和哺乳动物中MHC的进化提供基础。

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