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Rapid divergence of repetitive DNAs in Brassica relatives.

机译:甘蓝型油菜近缘种中重复DNA的快速分化。

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Centromeric, subtelomeric, and telomeric repetitive DNAs were characterized in Brassica species and the related Raphanus sativus and Arabidopsis thaliana. In general, rapid divergence of the repeats was found. The centromeric tandem satellite repeats were differentially distributed in the species studied, suggesting that centromeric repeats have diverged during the evolution of the A/C and B genome lineages. Sequence analysis of centromeric repeats suggested rapid evolution. Pericentromere-associated retrotransposons were identified and showed divergence during the evolution of the lineages as centromeric repeats. A novel subtelomeric tandem repeat from B. nigra was found to be conserved across the diploid Brassica genomes; however, this sequence was not identified in the related species. In contrast to previous studies, interstitial telomere-like repeats were identified in the pericentromeres of Brassica chromosomes, and these repeats may be associated with genomic stability. These results provide insight into genome evolution during polyploidization in Brassica and divergence within the Brassicaceae.
机译:芸苔属物种以及相关的萝卜(Raphanus sativus)和拟南芥(Arabidopsis thaliana)的特征在于着丝粒,亚端粒和端粒的重复DNA。通常,发现重复序列迅速分离。着丝粒串联卫星重复序列在所研究的物种中差异分布,表明着丝粒重复序列在A / C和B基因组谱系的进化过程中发生了分化。着丝粒重复序列的序列分析表明快速进化。鉴定了与周肠膜相关的逆转座子,并在着丝粒重复序列的谱系进化过程中显示出差异。发现来自黑芥子的新的亚端粒串联重复序列在芸苔二倍体基因组中是保守的。但是,该序列在相关物种中未鉴定。与以前的研究相比,在芸苔属染色体的着丝粒中发现了间质性端粒样重复序列,这些重复序列可能与基因组稳定性有关。这些结果提供了对芸苔属多倍体化过程和芸苔科内发散过程中基因组进化的见解。

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