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首页> 外文期刊>Genomics >Discovery of a functional retrotransposon of the murine phospholipid hydroperoxide glutathione peroxidase: chromosomal localization and tissue-specific expression pattern.
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Discovery of a functional retrotransposon of the murine phospholipid hydroperoxide glutathione peroxidase: chromosomal localization and tissue-specific expression pattern.

机译：鼠磷脂氢过氧化物谷胱甘肽过氧化物酶的功能性反转录转座子的发现：染色体定位和组织特异性表达模式。

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摘要

Phospholipid hydroperoxide glutathione peroxidase (PHGPx), a selenoprotein capable of reducing toxic hydroperoxy ester lipids, has been implicated in antioxidative defense and spermatogenesis. Screening a murine genomic library, we isolated two recombinants (pseudogenes 1 and 2) containing retrotransposons for this enzyme. On comparison with the paralogous cDNA, pseudogene 1 contained only two silent nucleotide exchanges, and the 3'-untranslated region (3'-UTR) carrying the functionally important selenocysteine insertion sequence was free of mutations. This retrotransposon was found in various mouse strains and could be mapped to the region B2-B3 of chromosome 10. In vitro studies indicated significant promoter activity in the 5'-flanking region of pseudogene 1, and we observed a tissuespecific expression of this retrotransposon. In the submandibular gland. Most PHGPx transcripts originated from pseudogene 1. In contrast, pseudogene 2, containing numerous mutations in all parts of the retrotransposon, was not expressed in any tissue. It was mapped to region E3-E4 of chromosome 17, and we did not detect any promoter activity in its 5'-flanking region. These data indicate the existence of two retrotransposed PHGPx pseudogenes, one of which encodes a functional enzyme. This retrotransposon belongs to the rare group of pseudogenes that are tissue-specifically expressed under the control of captured regulatory elements, and it constitutes an example of evolutionarily acquired redundancy in gene expression. The results are important for the design of future knockout strategies aimed at investigating the biological role of this enzyme.
机译：磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）是一种能够减少毒性氢过氧酯脂质的硒蛋白，与抗氧化防御和精子形成有关。筛选鼠类基因组文库，我们分离了两个含有该酶逆转座子的重组体（假基因1和2）。与旁系cDNA相比，假基因1仅包含两个沉默核苷酸交换，并且带有功能上重要的硒代半胱氨酸插入序列的3'非翻译区（3'-UTR）没有突变。该反转录转座子存在于多种小鼠品系中，可定位于10号染色体的B2-B3区域。体外研究表明，假基因1的5'侧翼区域具有明显的启动子活性，我们观察到了该反转录转座子的组织特异性表达。在下颌下腺中。大多数PHGPx转录物均来自假基因1。相反，在反转录转座子所有部分均包含许多突变的假基因2在任何组织中均未表达。它被定位到17号染色​​体的E3-E4区域，我们在其5'侧翼区域没有检测到任何启动子活性。这些数据表明存在两个逆转录转置的PHGPx假基因，其中一个编码功能性酶。该反转录转座子是在捕获的调节元件的控制下在组织中特异性表达的伪基因的罕见组，它构成了基因表达中进化获得的冗余的一个例子。该结果对于设计旨在研究该酶生物学作用的未来敲除策略非常重要。

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