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首页> 外文期刊>Genomics >A BAC-based physical map of the channel catfish genome.
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A BAC-based physical map of the channel catfish genome.

机译：channel鱼基因组的基于BAC的物理图。

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摘要

Catfish is the major aquaculture species in the United States. To enhance its genome studies involving genetic linkage and comparative mapping, a bacterial artificial chromosome (BAC) contig-based physical map of the channel catfish (Ictalurus punctatus) genome was generated using four-color fluorescence-based fingerprints. Fingerprints of 34,580 BAC clones (5.6x genome coverage) were generated for the FPC assembly of the BAC contigs. A total of 3307 contigs were assembled using a cutoff value of 1x10(-20). Each contig contains an average of 9.25 clones with an average size of 292 kb. The combined contig size for all contigs was 0.965 Gb, approximately the genome size of the channel catfish. The reliability of the contig assembly was assessed by both hybridization of gene probes to BAC clones contained in the fingerprinted assembly and validation of randomly selected contigs using overgo probes designed from BAC end sequences. The presented physical map should greatly enhance genome research in the catfish, particularly aiding in the identification of genomic regions containing genes underlying important performance traits.
机译：fish鱼是美国主要的水产养殖种类。为了增强其涉及遗传连锁和比较作图的基因组研究，使用基于四色荧光的指纹图生成了基于通道artificial鱼（Ictalurus punctatus）基因组的细菌人工染色体（BAC）重叠群的物理图谱。为BAC重叠群的FPC组装生成了34,580个BAC克隆的指纹（基因组覆盖率为5.6倍）。截止值为1x10（-20），总共组装了3307个重叠群。每个重叠群平均包含9.25个克隆，平均大小为292 kb。所有重叠群的合并重叠群大小为0.965 Gb，大约是channel鱼的基因组大小。重叠群装配的可靠性是通过将基因探针与指纹装配中包含的BAC克隆杂交，以及使用从BAC末端序列设计的过量探针验证随机选择的重叠群来评估的。提出的物理图谱应大大加强in鱼的基因组研究，特别是有助于鉴定包含潜在重要性状基因的基因组区域。

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