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首页> 外文期刊>Genomics >Directed sequencing and annotation of three Dicentrarchus labrax L. chromosomes by applying Sanger- and pyrosequencing technologies on pooled DNA of comparatively mapped BAC clones.
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Directed sequencing and annotation of three Dicentrarchus labrax L. chromosomes by applying Sanger- and pyrosequencing technologies on pooled DNA of comparatively mapped BAC clones.

机译：通过在比较定位的BAC克隆的合并DNA上应用Sanger-和焦磷酸测序技术，指导三个Dicentrarchus labrax L.染色体的测序和注释。

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Dicentrarchus labrax is one of the major marine aquaculture species in the European Union. In this study, we have developed a directed-sequencing strategy to sequence three sea bass chromosomes and compared results with other teleosts. Three BAC DNA pools were created from sea bass BAC clones that mapped to stickleback chromosomes/groups V, XVII and XXI. The pools were sequenced to 17-39x coverage by pyrosequencing. Data assembly was supported by Sanger reads and mate pair data and resulted in superscaffolds of 13.2 Mb, 17.5 Mb and 13.7 Mb respectively. Annotation features of the superscaffolds include 1477 genes. We analyzed size change of exon, intron and intergenic sequence between teleost species and deduced a simple model for the evolution of genome composition in teleost lineage. Combination of second generation sequencing technologies, Sanger sequencing and genome partitioning strategies allows "high-quality draft assemblies" of chromosome-sized superscaffolds, which are crucial for the prediction and annotation of complete genes.
机译：Dicentrarchus labrax是欧盟的主要海水养殖品种之一。在这项研究中，我们开发了一种定向测序策略来对三个鲈鱼染色体进行测序，并将结果与​​其他硬骨鱼进行比较。从鲈鱼BAC克隆创建了三个BAC DNA库，这些克隆映射到棘背染色体/ V，XVII和XXI组。通过焦磷酸测序将这些库测序至17-39x覆盖率。数据组装得到Sanger读取和配对对数据的支持，从而导致超级支架分别为13.2 Mb，17.5 Mb和13.7 Mb。超级支架的注释特征包括1477个基因。我们分析了硬骨鱼种之间外显子，内含子和基因间序列的大小变化，并推导了硬骨鱼谱系中基因组组成演变的简单模型。结合第二代测序技术，Sanger测序和基因组划分策略，可以实现染色体大小的超级支架的“高质量草图装配”，这对于预测和注释完整基因至关重要。

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