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首页> 外文期刊>Genomics >Generation of a complete set of human telomeric band painting probes by chromosome microdissection.
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Generation of a complete set of human telomeric band painting probes by chromosome microdissection.

机译：通过染色体显微切割生成完整的人类端粒带绘画探针集。

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摘要

Chromosomal rearrangements involving telomeric bands have been frequently detected in many malignancies and congenital diseases. To develop a useful tool to study chromosomal rearrangements within the telomeric band effectively and accurately, a whole set of telomeric band painting probes (TBP) has been generated by chromosome microdissection. The intensity and specificity of these TBPs have been tested by fluorescence in situ hybridization and all TBPs showed strong and specific signals to target regions. TBPs of 6q and 17p were successfully used to detect the loss of the terminal band of 6q in a hepatocellular carcinoma cell line and a complex translocation involving the 17p terminal band in a melanoma cell line. Meanwhile, the TBP of 21q was used to detect a de novo translocation, t(12;21), and the breakpoint at 21q was located at 21q22.2. Further application of these TBPs should greatly facilitate the cytogenetic analysis of complex chromosome rearrangements involving telomeric bands.
机译：在许多恶性肿瘤和先天性疾病中，经常发现涉及端粒带的染色体重排。为了开发一种有效，准确地研究端粒带内染色体重排的有用工具，通过染色体显微切割已经生成了一套完整的端粒带涂探探针（TBP）。这些TBP的强度和特异性已经通过荧光原位杂交进行了测试，并且所有TBP对靶区域均显示出强而特异性的信号。成功地将6q和17p的TBP用于检测肝细胞癌细胞系中6q末端带的缺失以及黑色素瘤细胞系中涉及17p末端带的复杂易位。同时，使用21q的TBP检测从头易位t（12; 21），并将21q的断点定位在21q22.2。这些TBP的进一步应用将极大地促进涉及端粒带的复杂染色体重排的细胞遗传学分析。

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