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Genotyping with TaqMAMA.

机译：TaqMAMA基因分型。

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摘要

TaqMAMA combines the quantitative strengths of TaqMan with the allele-specific PCR of MAMA. In this article we develop TaqMAMA as a technique for screening human DNA samples for known genetic polymorphisms. In the first set of experiments, plasmids that model all types of genetic polymorphisms were used to understand the relationship between TaqMAMA primer/template mismatches and their strength of allelic discrimination. These data can be used to improve allelic discrimination of other primer extension genotyping methodologies through directed use of nucleotide mismatches. We used the data to derive a guide for TaqMAMA primer design and DNA strand selection for TaqMAMA genotyping assays. The guide was then used to develop assays for 11 known and novel human genetic polymorphisms. Genotypes were assigned quickly and accurately in all cases. TaqMAMA genotyping assays require minimal development time, have a high probability of success, produce reliable data that are straightforward to analyze, and are very cost-competitive.
机译：TaqMAMA将TaqMan的定量优势与MAMA的等位基因特异性PCR相结合。在本文中，我们开发TaqMAMA作为一种筛选人类DNA样品中已知遗传多态性的技术。在第一组实验中，使用对所有类型的基因多态性建模的质粒来了解TaqMAMA引物/模板错配与其等位基因区分强度之间的关系。这些数据可用于通过定向使用核苷酸错配来改善其他引物延伸基因分型方法的等位基因鉴别。我们使用这些数据得出TaqMAMA引物设计指南和TaqMAMA基因分型分析的DNA链选择指南。然后，该指南用于开发11种已知和新颖的人类遗传多态性的检测方法。在所有情况下均快速，准确地确定了基因型。 TaqMAMA基因分型分析需要最少的开发时间，成功的可能性很高，产生的数据易于分析且具有成本竞争力。

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