Sequence analysis of genomic regions containing trinucleotide repeats isolated by a novel cloning method.

Inoue S; Takahashi K; Ohta M

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Sequence analysis of genomic regions containing trinucleotide repeats isolated by a novel cloning method.

机译：通过新型克隆方法分离的包含三核苷酸重复序列的基因组区域的序列分析。

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A method was developed for effective isolation of trinucleotide repeats from genomic DNA. This method is based on the DNA polymerase reaction, which is restricted with only two or three nucleotide substrates and primed by biotinylated oligonucleotide probes. Sequences are then isolated by a streptavidin biotin-trapping method. More than 80% of the clones from each library contained more than eight trinucleotide repeats. Sequence analysis showed that the characteristic dinucleotide flanking sequences usually confronting various trinucleotide repeats are not found in the vicinity of CAG repeats, suggesting that CAG repeats may have been generated through a mechanism different from that of other trinucleotide repeats. Copyright 1999 Academic Press.

机译：开发了一种从基因组DNA有效分离三核苷酸重复序列的方法。此方法基于DNA聚合酶反应，该反应仅受两个或三个核苷酸底物限制，并由生物素化的寡核苷酸探针引发。然后通过链霉亲和素生物素捕获方法分离序列。每个文库中超过80％的克隆包含八个以上的三核苷酸重复序列。序列分析表明，在CAG重复序列附近没有发现通常面对各种三核苷酸重复序列的特征性二核苷酸侧翼序列，这表明CAG重复序列可能是通过不同于其他三核苷酸重复序列的机制产生的。版权所有1999 Academic Press。

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《Genomics》 |1999年第1期|共4页
作者
Inoue S; Takahashi K; Ohta M;
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正文语种 eng
中图分类遗传学;
关键词
Cloning; Molecular; Trinucleotide Repeats; DNA Restriction Enzymes; 克隆; 分子; 三核苷酸重复;

机译：Cloning;Molecular;Trinucleotide Repeats;DNA Restriction Enzymes;克隆;分子;三核苷酸重复;

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1. Sequence analysis of genomic regions containing trinucleotide repeats isolated by a novel cloning method. [J] . Inoue S, Takahashi K, Ohta M Genomics . 1999,第1期

机译：通过新型克隆方法分离的包含三核苷酸重复序列的基因组区域的序列分析。
2. Analysis of the 5′ end of the rat plasma membrane Ca2+-ATPase isoform 3 gene and identification of extensive trinucleotide repeat sequences in the 5′ untranslated region [J] . Scott E. Burk, Anil G. Menon, Gary E. Shull Biochimica et biophysica acta. Biomembranes . 1995,第2期

机译：大鼠质膜Ca2 + -ATPase同工型3基因5'末端的分析和5'非翻译区中广泛的三核苷酸重复序列的鉴定
3. SINGLE-STRANDED DNA BINDING PROTEINS ISOLATED FROM MOUSE BRAIN RECOGNIZE SPECIFIC TRINUCLEOTIDE REPEAT SEQUENCES IN VITRO [J] . Yanoyanagisawa H, Li Y, Wang H, Nucleic Acids Research . 1995,第14期

机译：从小鼠脑中分离出的单链DNA结合蛋白可识别特定的三核苷酸重复序列
4. Recognizing Short Tandem Repeat Regions in Genomic Sequences Using Wavelet [C] . Padole Mamta C. International Conference on Mathematics and Computers in Sciences and in Industry . 2014

机译：使用小波识别基因组序列中的短串联重复序列区域
5. Identification of genomic clones and analysis of the promoter region for the human testis-specific lactate dehydrogenase. [D] . Brooke, Catherine Driscoll. 1991

机译：人类睾丸特异性乳酸脱氢酶的基因组克隆的鉴定和启动子区域的分析。
6. Single-stranded DNA binding proteins isolated from mouse brain recognize specific trinucleotide repeat sequences in vitro. [O] . H Yano-Yanagisawa, Y Li, H Wang, 1995

机译：从小鼠脑中分离出的单链DNA结合蛋白可在体外识别特定的三核苷酸重复序列。
7. Analysis of the 5′ end of the rat plasma membrane Ca2+-ATPase isoform 3 gene and identification of extensive trinucleotide repeat sequences in the 5′ untranslated region [O] . Burk Scott E., Menon Anil G., Shull Gary E. 1995

机译：大鼠质膜Ca2 + -ATPase同工型3基因5'末端的分析和5'非翻译区中广泛的三核苷酸重复序列的鉴定
8. Identification of Novel Inverted Terminal Repeat (ITR) Deletions of Human Adenovirus (AD) From Infected Host: Virulent Ads Containing Mixed Populations of Genomic Sequences; Conference paper [R] . Houng, H. H., Binn, L., Kuschner, R., 2006

机译：从受感染的宿主中鉴定新的人类腺病毒（aD）的倒置末端重复序列（ITR）：含有基因组序列的混合群体的病毒广告;会议论文

Sequence analysis of genomic regions containing trinucleotide repeats isolated by a novel cloning method.

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