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首页> 外文期刊>Genomics >The Gene CSTF2T, Encoding the Human Variant CstF-64 Polyadenylation Protein tauCstF-64, Lacks Introns and May Be Associated with Male Sterility.
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The Gene CSTF2T, Encoding the Human Variant CstF-64 Polyadenylation Protein tauCstF-64, Lacks Introns and May Be Associated with Male Sterility.

机译：编码人类变异CstF-64聚腺苷酸化蛋白tauCstF-64的基因CSTF2T，缺乏内含子，可能与雄性不育有关。

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Messenger RNA polyadenylation in male germ cells does not seem to require the AAUAAA polyadenylation signal required in all other cell types. To account for this difference, we found a variant form of the polyadenylation protein, the 64,000 Mr protein of the cleavage stimulation factor (CstF-64), in mouse meiotic and postmeiotic germ cells. This protein is a candidate to alter polyadenylation in those cells. More recently, we reported the cloning from mouse pachytene spermatocytes of mouse tauCstF-64 (gene symbol Cstf2t), which is a homolog of CstF-64 fitting the criteria we expected for the variant CstF-64 protein. Here we report the cloning and mapping of the human ortholog of mouse tauCstF-64. The human tauCstF-64 cDNA (gene symbol CSTF2T) is 2324 bp in length and encodes a protein of 616 amino acids (64,442.90 Da). Although most highly related to mouse tauCstF-64 (89.8% identity), human tauCstF-64 is also related to the human and mouse somatic CstF-64 (74.9% and 73.4% identity, respectively). Alignment of human tauCstF-64 with human genome sequence from chromosome 10 shows that CSTF2T lacks introns. Radiation hybrid mapping places the human tauCstF-64 gene at 10q22-q23, which is the site of a translocation that has been associated with human neurological problems and male infertility.
机译：雄性生殖细胞中的信使RNA聚腺苷酸似乎并不要求所有其他细胞类型都需要AAUAAA聚腺苷酸信号。为了解释这种差异，我们在小鼠减数分裂和减数分裂后的生殖细胞中发现了聚腺苷酸化蛋白的变体形式，即裂解刺激因子（CstF-64）的64,000 Mr蛋白。该蛋白是改变那些细胞中聚腺苷酸化的候选物。最近，我们报道了从小鼠tauCstF-64（基因符号Cstf2t）的小鼠粗线精子细胞克隆，这是CstF-64的同源物，符合我们预期的CstF-64蛋白变异标准。在这里，我们报告小鼠tauCstF-64人类直系同源基因的克隆和作图。人tauCstF-64 cDNA（基因符号CSTF2T）长2324 bp，编码616个氨基酸（64,442.90 Da）的蛋白质。尽管与小鼠tauCstF-64高度相关（同一性为89.8％），但人类tauCstF-64也与人和小鼠体细胞CstF-64相关（分别为74.9％和73.4％相同）。人类tauCstF-64与来自10号染色体的人类基因组序列的比对表明，CSTF2T缺乏内含子。辐射杂交作图将人类tauCstF-64基因置于10q22-q23，这是与人类神经系统疾病和男性不育症相关的易位部位。

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