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首页> 外文期刊>Genomics >The telomeric region of BTA18 containing a potential QTL region for health in cattle exhibits high similarity to the HSA19q region in humans small star, filled.
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The telomeric region of BTA18 containing a potential QTL region for health in cattle exhibits high similarity to the HSA19q region in humans small star, filled.

机译：BTA18的端粒区域包含一个潜在的牛健康QTL区域，与人类小恒星中的HSA19q区域高度相似。

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摘要

We have applied a targeted physical mapping approach, based on the isolation of bovine region-specific large-insert clones using homologous human sequences and chromosome microdissection, to enhance the physical gene map of the telomeric region of BTA18 and to prove its evolutionary conservation. The latter is a prerequisite to exploit the dense human gene map for future positional cloning approaches. Partial sequencing and homology search were used to characterize 20 BACs targeted to the BTA18q2.4-q2.6 region. We used fluorescence in situ hybridization (FISH) to create physical maps of 11 BACs containing 15 gene loci; these BACs served as anchor loci. Using these approaches, 12 new gene loci (CKM, STK13, PSCD2, IRF3, VASP, ACTN4, ITPKC, CYP2B6, FOSB, DMPK, MIA, SIX5) were assigned on BTA18 in the bovine cytogenetic map. A resolved physical map of BTA18q2.4-q2.6 was developed, which encompasses 28 marker loci and a comparative cytogenetic map that contains 15 genes. The mapping results demonstrate the high evolutionary conservation between the telomeric region of BTA18q and HSA19q.
机译：我们已经应用了针对性的物理作图方法，该方法基于使用同源人类序列和染色体显微切割技术分离牛区域特异性大插入克隆，以增强BTA18端粒区域的物理基因图谱并证明其进化保守性。后者是利用密集的人类基因图谱进行将来的位置克隆方法的先决条件。使用部分测序和同源性搜索来表征针对BTA18q2.4-q2.6区域的20个BAC。我们使用荧光原位杂交（FISH）创建了包含15个基因位点的11个BAC的物理图谱。这些BAC充当锚点。使用这些方法，在牛细胞遗传图谱中的BTA18上分配了12个新基因位点（CKM，STK13，PSCD2，IRF3，VASP，ACTN4，ITPKC，CYP2B6，FOSB，DMPK，MIA，SIX5）。解析的BTA18q2.4-q2.6物理图谱已开发出来，其中包括28个标记基因座和一个包含15个基因的比较细胞遗传学图谱。映射结果表明BTA18q和HSA19q的端粒区域之间的高度进化保守性。

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