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首页> 外文期刊>Genomics >Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2.
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Identification of the mouse paternally expressed imprinted gene Zdbf2 on chromosome 1 and its imprinted human homolog ZDBF2 on chromosome 2.

机译：小鼠父本在染色体1上表达的印迹基因Zdbf2及其在染色体2上的印迹人类同源物ZDBF2的鉴定。

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摘要

In mammals, both the maternal and paternal genomes are necessary for normal embryogenesis due to parent-specific epigenetic modification of the genome during gametogenesis, which leads to non-equivalent expression of imprinted genes from the maternal and paternal alleles. In this study, we identified a paternally expressed imprinted gene, Zdbf2, by microarray-based screening using parthenogenetic and normal embryos. Expression analyses showed that Zdbf2 was paternally expressed in various embryonic and adult tissues, except for the placenta and adult testis, which showed biallelic expression of the gene. We also identified a differentially methylated region (DMR) at 10 kb upstream of exon 1 of the Zdbf2 gene and this differential methylation was derived from the germline. Furthermore, we also identified that the human homolog (ZDBF2) of the mouse Zdbf2 gene showed paternal allele-specific expression in human lymphocytes but not in the human placenta. Thus, our findings defined mouse chromosome 1 and human chromosome 2 as the loci for imprinted genes.
机译：在哺乳动物中，由于配子发生过程中基因组的亲本特异性表观遗传学修饰，因此母本和父本基因组都是正常胚胎发生所必需的，这导致来自母本和父本等位基因的印迹基因的表达不等价。在这项研究中，我们通过使用孤雌生殖和正常胚胎的基于微阵列的筛选，鉴定了父本表达的印迹基因Zdbf2。表达分析表明，Zdbf2在各种胚胎和成年组织中父本表达，但胎盘和成年睾丸除外，后者显示了该基因的双等位基因表达。我们还鉴定了Zdbf2基因第1外显子上游10 kb处的甲基化差异区域（DMR），该甲基化差异来源于种系。此外，我们还确定了小鼠Zdbf2基因的人类同源物（ZDBF2）在人淋巴细胞中显示了父本等位基因特异性表达，但在人胎盘中未显示。因此，我们的发现将小鼠染色体1和人类染色体2定义为印迹基因的基因座。

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