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首页> 外文期刊>Genomics >Changes in the peripheral blood transcriptome associated with occupational benzene exposure identified by cross-comparison on two microarray platforms.
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Changes in the peripheral blood transcriptome associated with occupational benzene exposure identified by cross-comparison on two microarray platforms.

机译：在两个微阵列平台上通过交叉比较确定与职业性苯暴露相关的外周血转录组的变化。

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摘要

Benzene is an established cause of leukemia, and possibly lymphoma, in humans, but the underlying molecular pathways remain largely undetermined. We used two microarray platforms to identify global gene expression changes associated with well-characterized occupational benzene exposure in the peripheral blood mononuclear cells (PBMC) of a population of shoe-factory workers. Differential expression of 2692 genes (Affymetrix) and 1828 genes (Illumina) was found and the concordance was 50% (based on an average fold-change > or =1.3 from the two platforms), with similar expression ratios among the concordant genes. Four genes (CXCL16, ZNF331, JUN and PF4), which we previously identified by microarray and confirmed by real-time PCR, were among the top 100 genes identified by both platforms in the current study. Gene ontology analysis showed overrepresentation of genes involved in apoptosis among the concordant genes while pathway analysis identified pathways related to lipid metabolism. The two-platform approach allows for robust changes in the PBMC transcriptome of benzene-exposed individuals to be identified.
机译：苯是人类中白血病（可能是淋巴瘤）的既定原因，但基本的分子途径仍未确定。我们使用了两个微阵列平台来鉴定与制鞋工人的外周血单核细胞（PBMC）中特征明确的职业苯暴露相关的全局基因表达变化。发现了2692个基因（Affymetrix）和1828个基因（Illumina）的差异表达，并且一致性为50％（基于两个平台的平均折叠倍数>或= 1.3），一致基因之间的表达比率相似。我们先前通过微阵列鉴定并通过实时PCR确认的四个基因（CXCL16，ZNF331，JUN和PF4）在当前研究中被两个平台鉴定出的前100个基因中。基因本体论分析显示一致基因中参与凋亡的基因的过度表达，而通路分析则确定了与脂质代谢有关的通路。两平台方法可确定暴露于苯的个体的PBMC转录组的强大变化。

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