...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >A novel testis-specific metallothionein-like protein, tesmin, is an early marker of male germ cell differentiation.
【24h】

A novel testis-specific metallothionein-like protein, tesmin, is an early marker of male germ cell differentiation.

机译：一种新型的睾丸特异性金属硫蛋白样蛋白，tesmin，是雄性生殖细胞分化的早期标志。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have cloned a novel cDNA encoding testis-specific metallothionein-like protein, tesmin, by randomized RT-PCR on RNA from mouse tissues. Two tesmin-related transcripts (2.2 and 1.8 kb) in mouse and one (2.1 kb) in human were detected and cloned. These encode a cysteine-rich 32-kDa protein that contained a metallothionein-like motif. In situ hybridization analysis in adult mouse testis showed that tesmin is specifically expressed in spermatocytes. Quantitative RT-PCR at different stages of mouse postnatal development (days 4, 8, 12, 18, and 42) revealed that tesmin is expressed as early as day 8 and coincides with the entry of germ cells into meiosis. Furthermore, adult W/Wv sterile mice that harbor the c-kit mutation lacked tesmin expression. The gene is assigned to mouse chromosome 19B, which has been reported to translocate (11;19) in male sterile mice. Copyright 1999 Academic Press.
机译：我们已经通过对小鼠组织中RNA的随机RT-PCR克隆了编码睾丸特异性金属硫蛋白样蛋白tesmin的新型cDNA。检测并克隆了小鼠中的两个与特斯敏相关的转录本（2.2和1.8 kb）和人中的一个（2.1 kb）。它们编码富含半胱氨酸的32-kDa蛋白，其中包含金属硫蛋白样基序。成年小鼠睾丸的原位杂交分析表明，tesmin在精母细胞中特异性表达。小鼠出生后不同阶段（第4、8、12、18和42天）的定量RT-PCR显示，tesmin最早在第8天就表达出来，并且与生殖细胞进入减数分裂相吻合。此外，具有c-kit突变的成年W / Wv不育小鼠缺乏tesmin表达。该基因分配给小鼠染色体19B，据报道该染色体在雄性不育小鼠中易位（11; 19）。版权所有1999 Academic Press。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号