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首页> 外文期刊>Genes & Genetic Systems >Endogenous retrovirus-related sequences provide an alternative transcript of MCJ genes in human tissues and cancer cells
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Endogenous retrovirus-related sequences provide an alternative transcript of MCJ genes in human tissues and cancer cells

机译：内源性逆转录病毒相关序列提供了人类组织和癌细胞中MCJ基因的替代转录本

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摘要

The MCJ gene is a member of the DNAJ family, and its transcriptional event is controlled by methylation of the CpG island. In our study, we found LTR33 and LTR7 elements provided an alternative transcript within the MCJ gene. To detect different expression patterns between the originally reported MCJ transcript and the LTR-related transcript, we performed a RT-PCR approach using various human tissues and cancer cells. The original MCJ transcript was detected in human tissues and cancer cells, whereas the LTR-related transcript was only revealed in some cancer cells (HCT106, MCF-3, TE-1, Hela, and CCHM). We also performed a PCR analysis to compare the insertion lineage of the LTR elements with the genomic DNAs of primates, indicating that those LTR33 and LTR7 elements of HERV-H have been integrated into the primate genome at different times. Taken together, we suggest that HERV-related elements trigger transcriptome diversification during primate evolution.
机译：MCJ基因是DNAJ家族的成员，其转录事件由CpG岛的甲基化控制。在我们的研究中，我们发现LTR33和LTR7元件在MCJ基因内提供了另一种转录本。为了检测最初报告的MCJ转录本和LTR相关转录本之间的不同表达模式，我们使用各种人体组织和癌细胞进行了RT-PCR方法。在人组织和癌细胞中检测到了原始的MCJ转录本，而仅在某些癌细胞（HCT106，MCF-3，TE-1，Hela和CCHM）中发现了LTR相关的转录本。我们还进行了PCR分析，以比较LTR元素的插入谱系与灵长类动物的基因组DNA，表明HERV-H的LTR33和LTR7元素已在不同时间整合到灵长类动物基因组中。两者合计，我们建议与HERV相关的元素触发灵长类动物进化过程中的转录组多样化。

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