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首页> 外文期刊>Genes & Genetic Systems >Complete assignment of structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of the shallot (Allium cepa L.).
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Complete assignment of structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of the shallot (Allium cepa L.).

机译：将影响灯泡颜色的类黄酮生物合成中涉及的结构基因完全分配给青葱的单个染色体（葱属）。

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摘要

We analyzed Japanese bunching onion (Allium fistulosum L.) - shallot (Allium cepa L. Aggregatum group) alien chromosome addition lines in order to assign the genes involved in the flavonoid biosynthesis pathway to chromosomes of the shallot. Two complete sets of alien monosomic additions (2n=2x+1=17) were used for determining the chromosomal locations of several partial sequences of candidate genes, CHS, CHI, F3H, DFR, and ANS via analyses of PCR-based markers. The results of DNA marker analyses showed that the CHS-A, CHS-B, CHI, F3H, DFR, and ANS genes should be assigned to chromosomes 2A, 4A, 3A, 3A, 7A, and 4A, respectively. HPLC analyses of 14 A. fistulosum - shallot multiple alien additions (2n=2x+2-2x+7=18-23) were conducted to identify the anthocyanin compounds produced in the scaly leaves. A direct comparison between the genomic constitution and the anthocyanin compositions of the multiple additions revealed that a 3GT gene for glucosylation of anthocyanidin was located on 4A. Thus, we were able to assign all structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of A. cepa..
机译：我们分析了日本聚束洋葱（Allist fistulosum L。）-青葱（Allium cepa L. Aggregatum组）外来染色体添加系，以便将参与类黄酮生物合成途径的基因分配给青葱的染色体。通过基于PCR的标记分析，使用两套完整的外来单体添加物（2n = 2x + 1 = 17）确定候选基因CHS，CHI，F3H，DFR和ANS的几个部分序列的染色体位置。 DNA标记分析的结果表明，CHS-A，CHS-B，CHI，F3H，DFR和ANS基因应分别分配给2A，4A，3A，3A，7A和4A染色体。进行了14种瘘管-葱的多种外来添加物（2n = 2x + 2-2x + 7 = 18-23）的HPLC分析，以鉴定鳞状叶片中产生的花色苷化合物。在多次添加的基因组组成和花青素组成之间的直接比较显示，花青素糖基化的3GT基因位于4A上。因此，我们能够将参与影响黄酮颜色的类黄酮生物合成的所有结构基因分配给洋葱曲霉的各个染色体。

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