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首页> 外文期刊>Genes & Genetic Systems >Involvement of OS-2 AUT kinase in regulation of the large-subunit catalases CAT-1 and CAT-3 in Neurospora crassa
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Involvement of OS-2 AUT kinase in regulation of the large-subunit catalases CAT-1 and CAT-3 in Neurospora crassa

机译：OS-2 AUT激酶参与神经孢菌大亚基过氧化氢酶CAT-1和CAT-3的调控

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摘要

Neurospora crassa has four catalase genes - cat-1, cat-2, cat-3, and ctt-1/cat-4. cat-1 and cat-3 encode two fungal-specific large-subunit catalases CAT-1 and CAT-3 normally produced in conidia and growing hyphae, respectively. cat-2 encodes CAT-2 catalase-peroxidase normally produced in conidia. ctt-1 (or cat-4), of which expression was controlled by OS-2 MAP kinase (Noguchi et al., Fungal Genet. Biol. 44, 208-218), encodes a small-subunit catalase with unknown function. To clarify the contribution of OS-2 on the regulation of CAT-1, CAT-2, and CAT-3, we performed quantitative RT-PCR and in-gel catalase activity analyses. When the hyphae were treated with a fungicide (1 [mu g/ml fludioxonil) or subjected to an osmotic stress (1 M sorbitol), cat-1 was strongly upregulated and CAT-1 was reasonably induced in the wild-type strain. Interestingly, fludioxonil caused not only the CAT-1 induction but also a remarkable CAT-3 decrease in the wild-type hyphae, implying of an abnormal stimulation of asexual differentiation. These responses were not observed in an os-2 mutant hyphae, indicating an involvement of OS-2 in the cat-1 expression; however, os-2 was dispensable for the production of CAT-1 in conidia. In contrast, the expression of cat-2 was significantly induced by heat shock (45 degrees C) and that of cat-3 was moderately stimulated by an oxidative stress (50 mu g/ml methyl viologen) in both the wild-type strain and the os-2 mutant, and corresponding enzyme activities were detected after the treatments. Although basal levels of transcription of cat-1 and cat-3 in an os-2 mutant hyphae were a few-fold lower than in the wild-type hyphae, the os-2 mutant exhibited a considerably lower levels of CAT-3 activity than the wild-type strain. These findings suggest that OS-2 MAP kinase regulated the expression of cat-1 and cat-3 transcriptionally, and probably that of cat-3 posttranscriptionally, even though the presence of another regulatory system for each of these two genes is evident.
机译：芥菜神经孢菌具有四个过氧化氢酶基因-cat-1，cat-2，cat-3和ctt-1 / cat-4。 cat-1和cat-3分别编码通常在分生孢子和生长菌丝中产生的两种真菌特异性大亚基过氧化氢酶CAT-1和CAT-3。 cat-2编码通常在分生孢子中产生的CAT-2过氧化氢酶过氧化物酶。 ctt-1（或cat-4）的表达受OS-2 MAP激酶控制（Noguchi等人，Fungal Genet。Biol。44，208-218），其编码的小亚基过氧化氢酶功能未知。为了阐明OS-2对CAT-1，CAT-2和CAT-3调控的贡献，我们进行了定量RT-PCR和凝胶内过氧化氢酶活性分析。当菌丝用杀菌剂（1μg / ml氟地西尼）处理或受到渗透压（1 M山梨糖醇）时，cat-1强烈上调，并且在野生型菌株中合理诱导了CAT-1。有趣的是，氟地西尼不仅引起CAT-1的诱导，而且引起野生型菌丝的CAT-3明显减少，这暗示着对无性分化的异常刺激。在os-2突变菌丝中未观察到这些反应，表明OS-2与cat-1表达有关。但是，os-2对于分生孢子中CAT-1的产生是必不可少的。相反，在野生型和野生型菌株中，热激（45摄氏度）显着诱导cat-2的表达，而氧化应激（50μg / ml甲基紫精）适度刺激cat-3的表达。处理后，检测到os-2突变体和相应的酶活性。尽管os-2突变菌丝中cat-1和cat-3的基础转录水平比野生型菌丝低几倍，但os-2突变体的CAT-3活性水平却大大低于野生型菌丝。野生型菌株。这些发现表明，OS-2 MAP激酶在转录后调节cat-1和cat-3的表达，并可能在转录后调节cat-3的表达，即使这两个基因中的每一个均存在另一个调控系统也是如此。

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