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首页> 外文期刊>Genes & Genetic Systems >Genome-wide expression profiling and identification of genes under the control of the DROOPING LEAF gene during midrib development in rice.
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Genome-wide expression profiling and identification of genes under the control of the DROOPING LEAF gene during midrib development in rice.

机译：水稻中脉发育过程中DROOPING LEAF基因控制下的全基因组表达谱分析和鉴定。

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摘要

The DROOPING LEAF (DL) gene has an important function in rice development. Loss-of-function mutation of DL fails to form midrib, a strong structure formed in the central region of the leaf, resulting in a drooping leaf phenotype. In addition, severe mutation in DL causes a floral homeotic change, i.e., replacement of the carpels by the stamens. Thus, DL regulates midrib formation in the leaf and carpel specification in the flower. DL encodes a putative transcription factor that belongs to the YABBY family, suggesting that it plays an essential role in these developmental processes by regulating many downstream genes, either directly or indirectly. As a first step to elucidate genetic networks involved in midrib formation, in this paper, we attempt to identify genes regulated by DL in the initial processes of midrib development. By comparing expression profiles in the shoot apex that involves the leaf primordia, we found 90 genes that are upregulated at least two-fold higher in the transgenic plants overexpressing DL (Act1:DL) than in the null dl mutant (dl-sup1), suggesting that these genes are involved in midrib formation under the control of DL. Subsequent RT-PCR analysis of arbitrarily chosen 18 genes suggests that some genes may be involved in only midrib formation, whereas others may be involved in both midrib formation and carpel development.
机译：DROOPING LEAF（DL）基因在水稻发育中具有重要作用。 DL的功能丧失突变未能形成中脉，中脉是在叶中央区域形成的牢固结构，导致叶片下垂。另外，DL中的严重突变引起花的同种异体变化，即用雄蕊替代心皮。因此，DL调节叶片中叶的中脉形成和花朵中的心皮规格。 DL编码属于YABBY家族的推定转录因子，表明它通过直接或间接调节许多下游基因在这些发育过程中起着至关重要的作用。作为阐明中脉形成过程中涉及的遗传网络的第一步，我们试图在中脉发育的初始过程中鉴定由DL调控的基因。通过比较涉及叶原基的茎尖中的表达谱，我们发现过表达DL（Act1：DL）的转基因植物中90个基因的上调至少是无效dl突变体（dl-sup1）的两倍，提示这些基因在DL的控制下参与了中脉的形成。随后对任意选择的18个基因进行RT-PCR分析表明，某些基因可能仅与中脉形成有关，而另一些基因可能与中脉形成和心皮发育有关。

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