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首页> 外文期刊>Genes & Genetic Systems >Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana.
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Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana.

机译：3-酮基-ACP合酶2基因在拟南芥中的表达和发育功能。

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摘要

The 3-ketoacyl-ACP synthase (KAS) II is a fatty-acid-related enzyme which catalyzes the elongation of 16:0-acyl carrier protein (ACP) to 18:0-ACP in plastids. The fatty acid biosynthesis 1-1 (fab1-1) mutant of Arabidopsis thaliana is partially deficient in its activity of Arabidopsis thaliana 3-ketoacyl-ACP synthase 2 (AtKAS2), and its phenotype has been intensively studied in connection with the chilling resistance and fatty acid composition. In this study, we used the T-DNA insertion mutant of AtKAS2 to examine its possible role in plant development. Reverse transcription (RT)-PCR showed that the AtKAS2 gene was expressed in various plant organs, except for roots, and was highly expressed in siliques. The fusion of beta-glucuronidase (GUS) to the AtKAS2 promoter demonstrated that the promoter was active in various tissues such as embryos, stomatal guard cells, inflorescences and pollen grains. We were not able to identify atkas2 homozygous mutant adult plants in heterozygous mutant progeny. Phenotypic and genetic analyses showed that disruption of the AtKAS2 by T-DNA insertion caused embryo lethality, and the development of the embryos was arrested at the globular stage. Taken together, our results suggest that AtKAS2 is required for embryo development in Arabidopsis during the transition from the globular to the heart stage.
机译：3-酮酰基-ACP合酶（KAS）II是一种脂肪酸相关的酶，可催化质体中16：0-酰基载体蛋白（ACP）延伸至18：0-ACP。拟南芥的脂肪酸生物合成1-1（fab1-1）突变体部分缺乏拟南芥3-酮酰基-ACP合酶2（AtKAS2）的活性，并且已对其表型进行了深入研究，涉及耐寒性和抗寒性。脂肪酸组成。在这项研究中，我们使用AtKAS2的T-DNA插入突变体来检查其在植物发育中的可能作用。逆转录（RT）-PCR显示，AtKAS2基因在除根部外的各种植物器官中均有表达，并在长角果中高表达。 β-葡糖醛酸糖苷酶（GUS）与AtKAS2启动子的融合表明该启动子在各种组织（如胚胎，气孔保卫细胞，花序和花粉粒）中均具有活性。我们无法在杂合突变体后代中鉴定atkas2纯合突变体成年植物。表型和遗传分析表明，通过T-DNA插入破坏AtKAS2会导致胚胎致死，并且胚胎的发育在球状阶段被阻止。两者合计，我们的结果表明AtKAS2是拟南芥从球状到心脏阶段过渡过程中胚胎发育所必需的。

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