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首页> 外文期刊>Genes & Genetic Systems >New SINE families from rice, OsSN, with poly(A) at the 3' ends.
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New SINE families from rice, OsSN, with poly(A) at the 3' ends.

机译：来自水稻OsSN的新SINE家族，其3'末端带有poly（A）。

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A database search of the sequences flanking a member of rice retrotransposon RIRE7 revealed that a 298-bp sequence in the region downstream of the member is a repetitive sequence interspersed in the genome of Oryza sativa cv. Nipponbare. Most of the repetitive sequences were flanked by a direct repeat of a target-site sequence, about 14 bp in length. The consensus sequence, 293 bp in length, had no regions encoding any proteins but had sequence motifs of an internal promoter of RNA polymerase III. These indicate that the sequence is a retroposon SINE, designated OsSN1 (Oryza sativa SINE1). OsSN1 is a new rice SINE, because it has no homology with any of the three p-SINE families previously identified from rice, and because it has a stretch of A at the 3' end, unlike p-SINE and any other Gramineae SINEs which have a stretch of T at the 3' end. The Nipponbare genome was found to have many members related to OsSN1, forming two additional new SINE families (designated OsSN2 and OsSN3). OsSN2 and OsSN3 are highly homologous to the 3' and 5' regions of OsSN1, respectively. This suggests that OsSN1 has a mosaic structure, which is generated by sequence exchange (or shuffling) between ancestral OsSN2 and OsSN3. Despite the absence of homology in the 3' regions between OsSN1 (or OsSN2) and OsSN3, a sequence, 5'-TTCTC-3', is commonly present in the region preceding the A stretch at the 3' end. This sequence together with the A stretch and a stem-loop structure found in the region near the A stretch are assumed to be important for retroposition. OsSN members were present in strains of Oryza species, as were p-SINE members. Some of the members showed insertion polymorphism at the respective loci among the rice strains. p-SINE had such polymorphic members, which are useful for classification and phylogenetic analysis of various strains of Oryza species. The polymorphic members of OsSN were more frequently found than those of p-SINE, and therefore, such members are likely to be useful for extensive taxonomic and phylogenetic studies on various rice strains.
机译：对水稻反转录转座子RIRE7成员侧翼序列的数据库搜索显示，该成员下游区域中一个298bp的序列是穿插在水稻基因组中的重复序列。 Nipponbare。大多数重复序列的侧翼是靶位点序列的直接重复，长度约为14 bp。共有序列长293bp，没有编码任何蛋白质的区域，但是具有RNA聚合酶III内部启动子的序列基序。这些表明该序列是反转录子SINE，称为OsSN1（Oryza sativa SINE1）。 OsSN1是一种新的水稻SINE，因为它与先前从水稻中鉴定出的三个p-SINE家族都不具有同源性，并且因为它在3'端具有一个A延伸序列，这与p-SINE和任何其他禾本科SINE不同。在3'端有一段T。发现Nipponbare基因组具有许多与OsSN1相关的成员，形成了另外两个新的SINE家族（称为OsSN2和OsSN3）。 OsSN2和OsSN3分别与OsSN1的3'和5'区域高度同源。这表明OsSN1具有镶嵌结构，该结构是通过祖先OsSN2和OsSN3之间的序列交换（或改组）生成的。尽管在OsSN1（或OsSN2）和OsSN3的3'区域中没有同源性，序列5'-TTCTC-3'通常出现在3'端A序列之前的区域中。假定该序列以及A延伸和在A延伸附近区域发现的茎环结构对于逆向定位很重要。 OsSN成员存在于Oryza菌株中，p-SINE成员也存在。一些成员在水稻品系的各个基因座处表现出插入多态性。 p-SINE具有这种多态性成员，可用于对Oryza种的各种菌株进行分类和系统发育分析。 OsSN的多态性成员比p-SINE的多态性成员更常见，因此，此类成员可能可用于对各种水稻菌株进行广泛的分类学和系统发育研究。

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