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首页> 外文期刊>Genes & Genetic Systems >Behavioral analyses of mutants for two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila melanogaster.
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Behavioral analyses of mutants for two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila melanogaster.

机译：果蝇中两个气味结合蛋白基因Obp57d和Obp57e突变体的行为分析。

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摘要

The odorant-binding protein (OBP) functions in chemosensation in insects. Two OBP genes, Obp57d and Obp57e, are involved in the evolution of the unique host-plant preference in Drosophila sechellia. Comparative analysis of the Obp57d/e genomic sequences in the Drosophila melanogaster species group has revealed that the rapid evolution of Obp57d and Obp57e has resulted in functional divergence between the two genes. Here, using D. melanogaster knockout strains generated by gene targeting, we examined the roles of Obp57d and Obp57e in behavioral response to a series of fatty acids. In the taste-based oviposition-site preference assay, the knockout flies showed stronger preference for acids than wild-type flies, indicating that the normal functions of Obp57d and Obp57e are associated with the suppression of positive preference for C6-C9 acids. Heterozygotes for each knockout haplotype also exhibited a significantly different behavioral response compared with wild-type flies, suggesting that Obp57d and Obp57e have a gene dosage effect on behavior. In contrast, the wild-type and knockout flies exhibited similar responses in the feeding assay and the odor-based free-walking assay, suggesting that the two OBPs' contribution to feeding and olfactory behaviors is small. Taken together, our results demonstrated that each of Obp57d and Obp57e in D. melanogaster contributes to the determination of reproductive sites, suggesting that the two OBP genes play an important role in ecological adaptation of Drosophila.
机译：气味结合蛋白（OBP）在昆虫的化学传感中起作用。果蝇的两个独特的OBP基因Obp57d和Obp57e参与了独特的寄主植物偏好的进化。果蝇果蝇物种组中Obp57d / e基因组序列的比较分析表明，Obp57d和Obp57e的快速进化导致了这两个基因之间的功能差异。在这里，我们使用通过基因定位产生的D. melanogaster敲除菌株，我们研究了Obp57d和Obp57e在对一系列脂肪酸的行为响应中的作用。在基于味觉的产卵位点偏爱分析中，基因敲除果蝇显示出比野生型果蝇对酸更强的偏爱，表明Obp57d和Obp57e的正常功能与抑制C6-C9酸的阳性偏爱有关。与野生型果蝇相比，每种敲除单倍型的杂合子还表现出显着不同的行为反应，这表明Obp57d和Obp57e对行为具有基因剂量效应。相比之下，野生型和敲除蝇在饲喂试验和基于气味的自由行走试验中表现出相似的响应，这表明两个OBP对饲喂和嗅觉行为的贡献很小。两者合计，我们的结果表明，黑腹果蝇中的Obp57d和Obp57e均有助于确定生殖部位，这表明这两个OBP基因在果蝇的生态适应中起着重要作用。

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