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首页> 外文期刊>Genes & Genetic Systems >Identification of small RNAs in late developmental stage of rice anthers.
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Identification of small RNAs in late developmental stage of rice anthers.

机译：水稻花药发育后期小RNA的鉴定。

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Small RNAs including microRNA (miRNA) and small interfering RNA (siRNA) are known as repressors of gene expression. There are many plant proteins involved in small RNA-mediated gene silencing, such as Dicer ribonucleases and RNA-dependent RNA polymerases. However, most of these proteins have been reported to be absent in the late developmental stage of the plant male gamete, pollen. In order to clarify the existence of the small RNAs during maturation of pollen, we cloned and sequenced small RNAs from rice anthers including tricellular pollen. From fifty six candidates of small RNAs, we identified two known miRNAs (miR166 and miR167), eight potential miRNAs, and ten putative heterochromatic siRNAs (hc-siRNAs). RNA gel blot analyses clearly showed that miR166 and miR167 were accumulated in the uninuclear pollen stage of anther development and remained until the tricellular pollen stage. Our cloning and RNA gel blot analyses of small RNAs led us to propose a possible function of small RNA-mediated gene regulation for the development of male gametes in rice.
机译：包括microRNA（miRNA）和小干扰RNA（siRNA）在内的小RNA被称为基因表达的阻遏物。有许多植物蛋白参与小RNA介导的基因沉默，例如Dicer核糖核酸酶和RNA依赖性RNA聚合酶。然而，据报道，大多数这些蛋白质在植物雄配子花粉的发育后期都不存在。为了阐明花粉成熟过程中小RNA的存在，我们从水稻花药（包括三细胞花粉）中克隆并测序了小RNA。从五十六个小RNA候选物中，我们鉴定出两个已知的miRNA（miR166和miR167），八个潜在的miRNA和十个推定的异色siRNA（hc-siRNA）。 RNA凝胶印迹分析清楚地表明，miR166和miR167积累在花药发育的单核花粉阶段，一直保持到三细胞花粉阶段。我们对小RNA的克隆和RNA凝胶印迹分析使我们提出了小RNA介导的基因调控对水稻雄性配子发育的可能作用。

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