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首页> 外文期刊>Genes & Genetic Systems >The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae species.
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The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae species.

机译：十字花科物种Ty1-copia和Ty3-吉普赛逆转座子的扩增和分化模式。

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摘要

One of the causes of genome size expansion is considered to be amplification of retrotransposons. We determined nucleotide sequences of 24 PCR products for each of six retrotransposons in Brassica rapa and Brassica oleracea. Phylogenetic trees of these sequences showed species-specific clades. We also sequenced STF7a homologs and Tto1 homologs, 24 PCR products each, in nine diploids and three allopolyploids, and constructed phylogenetic trees. In these phylogenetic trees, species-specific clades of diploid species were also formed, but retrotransposons of allopolyploids were clustered into the clades of their original genomes, indicating that these two retrotransposons amplified after speciation of the nine diploids. Genetic variation in these retrotransposons may have arisen before emergence of allopolyploid species. There was a positive correlation between the genome size and the average number of substitutions of STF7a and Tto1 homologs in at least seven diploids. The implications of these results in the genome evolution of Brassicaceae are herein discussed.
机译：基因组大小扩大的原因之一被认为是反转录转座子的扩增。我们为甘蓝型油菜和甘蓝型油菜中的六个反转录转座子分别确定了24种PCR产物的核苷酸序列。这些序列的系统发育树显示出物种特异性进化枝。我们还对9个二倍体和3个异源多倍体中的STF7a同源物和Tto1同源物进行了测序，每个都有24个PCR产物，并构建了系统树。在这些系统发育树中，还形成了二倍体物种的种属特异性进化枝，但是同素多倍体的反转录转座子聚集在其原始基因组的进化枝中，表明这两个反转录转座子在对九个二倍体进行了物种形成后得到了扩增。这些逆转座子的遗传变异可能是在异源多倍体物种出现之前出现的。在至少七个二倍体中，基因组大小与STF7a和Tto1同系物的平均取代数之间存在正相关。本文讨论了这些结果对十字花科的基因组进化的影响。

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