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Degradation of Nucleosides by Direct and Alternating Electric Currents

机译：直流电和交流电对核苷的降解

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Degradation of four nucleosides, 2'-deoxyguanosine, 2'-deoxyadenosine, 2'-deoxycytidine, and thymidine, in potassium phosphate buffer of pH 7.4 by direct and alternating electric currents was monitored by HPLC. When a direct current of 3 V was applied to the nucleoside solution, all the nucleosides were consumed time-dependently. Results for voltage dependence showed that the nucleosides were decomposed above 0.8 V for 2'-deoxyguanosine, 1.5 V for 2'-deoxyadenosine, and 2.5 V for 2'-deoxy-cytidine and thymidine. Results of frequency dependence of alternating current at 3 V showed that increasing frequency caused decreasing consumption of all the nucleosides, and that no consumption was observed above 3,000 Hz. The results suggest that accidental ingestion of batteries or electrical shock by domestic power supply may induce alternation of nucleosides.
机译：通过HPLC监测在pH 7.4的磷酸钾缓冲液中通过直流电和交流电对四种核苷2'-脱氧鸟苷，2'-脱氧腺苷，2'-脱氧胞苷和胸苷的降解。当向核苷溶液施加3 V直流电时，所有核苷均被时间消耗。电压依赖性的结果表明，对于2'-脱氧鸟苷而言，核苷在0.8 V以上分解，对于2'-脱氧腺苷，则为1.5 V，对于2'-脱氧胞苷和胸苷为2.5V。 3 V交流电的频率依赖性结果表明，增加频率会导致所有核苷的消耗减少，并且在3,000 Hz以上未观察到消耗。结果表明，家用电源意外摄入电池或触电可能会引起核苷的交替。

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