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Organization of the 5S rRNA gene units in Korean Lilium species

机译：韩国百合属物种中5S rRNA基因单位的组织

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摘要

The 5S rRNA gene unit was characterized from Lilium species distributed in Korea. Sequence analyses revealed that the 5S rDNA, harboring a highly conserved coding region (120 bp) and a divergent non transcribed spacer (NTS), ranged from 470 bp to 640 bp. The NTS regions showed length heterogeneity (350 bp-520 bp) due to the presence of several indels, and extensive sequence divergence among the species. Despite heterogeneity in length and nucleotide composition, the NTS regions possess some common features across the species, which include a T stretch region CTTTT and an identical motif of 11 bp (CAATGTATGAC) at the 3' and 5' flanking sequence of the coding region, respectively. These may play a role in the initiation and termination of transcription. The chromosomal distribution of the 5S rRNA gene was mapped on the long arm of chromosome 3 by fluorescence in situ hybridization (FISH). The phylogenetic analysis based on the NTS sequence broadly divided the Lilium species into two major groups which were referred to as the section Sinomartagon and Martagon by Comber's classification of the genus Lilium. The present study showed that the 5S rDNA sequence was very useful to unravel the genetic relationships among Lilium species.
机译：从韩国分布的百合属植物中鉴定了5S rRNA基因单元。序列分析表明，具有高度保守的编码区（120 bp）和发散的非转录间隔区（NTS）的5S rDNA的范围为470 bp至640 bp。由于存在几个插入缺失，以及物种间广泛的序列差异，NTS区显示出长度异质性（350 bp-520 bp）。尽管长度和核苷酸组成存在异质性，NTS区在整个物种中仍具有一些共同特征，其中包括T延伸区CTTTT和编码区3'和5'侧翼序列的11 bp相同基序（CAATGTATGAC），分别。这些可能在转录的起始和终止中起作用。通过荧光原位杂交（FISH）将5S rRNA基因的染色体分布定位在3号染色体的长臂上。基于NTS序列的系统发育分析将百合属物种大致分为两大类，根据康伯对百合属的分类，将其称为中华mart和and节。本研究表明5S rDNA序列对于揭示百合属植物之间的遗传关系非常有用。

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